摘要
目的:探讨miR-200c在子宫内膜癌组织中的表达及与ZEB2的相关性。方法:选取113例子宫内膜癌组织和癌旁组织标本,40例正常子宫内膜癌组织作为对照组,检测组织中miR-200c表达量,以及ZEB2基因和蛋白表达量,利用反义寡核苷酸技术抑制子宫内膜癌RL95-2细胞株中miR-200c表达,检测其对细胞侵袭力的影响,以及细胞中ZEB2基因和蛋白表达。结果:子宫内膜癌组织中miR-200c表达量、ZEB2基因和蛋白表达量均高于癌旁组织和对照组(P<0.05);反义miR-200c转染组穿膜细胞数少于阴性对照组和脂质体组(P<0.05);反义miR-200c转染组细胞中ZEB2基因和蛋白表达量均低于阴性对照组和脂质体组(P<0.05)。结论 :miR-200c在子宫内膜癌组织中呈高表达,可能是通过调控ZEB2而促进肿瘤细胞的浸润和转移。
Objective To investigate the expression of miR-200c in endometrial carcinoma and its correlation with ZEB2 protein. Methods The expression levels of miR-200c and ZEB2 gene and protein in tissues were detected. The expression of miR-200c in endometrial carcinoma RL95-2 cell line was inhibited by using antisense oligonueleotides and its effect on cell invasiveness was tested. The expressions of ZEB2 gene and protein in cells were detected. Restdts The expression levels of miR-200c and ZEB2 mRNA and protein in endometrial tissues were significantly higher than those in adjacent tissues and control group （P 〈 0.05）. The average penetrating number in antisense miR-200c transfected group was significantly less than negative control group and liposome group （P 〈 0.05）. The expression levels of ZEB2 gene and protein in antisense miR-200c transfected group were lower than the negative control group and liposome group （P 〈 0.05）. Conclusion The expression of mRNA-200c in endometrial carcinoma was high and it might be promoting tumor cell invasion and metastasis by regulating ZEB2.
作者
苏纯
李巧云
张薇
SU Chun LI Qiao-yun ZHANG Wei.(Department of Gynecology and Obstetrics, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Chin)
出处
《实用医学杂志》
CAS
北大核心
2016年第18期3057-3059,共3页
The Journal of Practical Medicine
