摘要
采用正交设计和响应面分析优化种子罐培养基配方,以菌体浓度、淀粉酶活力和黏度为指标,得到优化培养基配方:黄豆饼粉3.0%,硫酸铵0.15%,玉米浆0.57%,玉米淀粉1.81%,糊精2.37%,葡萄糖1.0%,豆油1.0%,氯化钠0.5%,碳酸钙0.4%,消泡剂0.02%。应用优化后的培养基进行15 L种子罐发酵培养,获得了更高质量的种子。将该种子接种至50 L发酵罐,红霉素A的发酵产量由8 057?g/ml提高到8 563?g/ml,提高了6.0%。
An orthogonal design and a response surface methodology were used to optimize the seed cultivation conditions, which was evaluated by mycelium concentration, amylase activity and viscosity. The optimized medium was determined as follows： soybean meal 3.0%, （NH4）2SO4 0.15%, corn steep liquor 0.57%, starch 1.81%, dextrin 2.37%, glucose 1.0%, soybean oil 1.0%, NaCl 0.5%, CaCO3 0.4%, and antifoam 0.02%. The higher quality seed was obtained using the optimized medium, and then was inoculated into a 50 L fermenter. As a result, the erythromycin A concentration was increased from 8 057 μg/ml to 8 563 μg/ml, a 6.0% increase compared with the original seed.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2016年第10期1245-1250,共6页
Chinese Journal of Pharmaceuticals
关键词
红霉素
正交设计
响应面分析
发酵培养
erythromycin
orthogonal design
response surface methodology
fermentation
