摘要
建立了高效液相色谱法同时测定盐酸达卡他韦及其5个光学异构体。采用Chiralpak IC色谱柱,以甲基叔丁醚（含0.1%二乙胺）∶甲醇（88∶12）为流动相,检测波长305 nm。盐酸达卡他韦及其5个光学异构体均分离完全,在0.1-4μg/ml范围内线性关系良好。方法回收率〉99.0%,RSD〈1.5%。
An HPLC method was established for the determination of daclatasvir dihydrochloride and its five enantiomers. A Chiralpak IC column was used, with the mobile phase of methyl tertbutyl ether （containing 0.1% diethylamine）∶ethanol （88∶12） at the detection wavelength of 305 nm. Daclatasvir dihydrochloride and its enantiomers could be separated successfully. It was linear for the above compounds in the range of 0.1—4 μg/ml. Their recoveries were over 99.0%, with RSDs less than 1.5%.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2016年第10期1304-1307,共4页
Chinese Journal of Pharmaceuticals
基金
科技部“十二五”重大新药创制科技重大专项(2013ZX09402103)
关键词
盐酸达卡他韦
光学异构体
高效液相色谱
拆分
含量测定
daclatasvir dihydrochloride
enantiomer
HPLC
chrial separation
content determination
