摘要
目的评估肺炎支原体载量指数(MPLI)与血清总抗体在MP肺炎诊断中的价值。方法选取115例肺炎支原体肺炎患儿和400例健康儿童为研究对象,荧光定量PCR与被动凝集法检测MP肺炎患儿不同病程阶段的MP-DNA及总抗体水平,并计算MPLI。结果肺炎支原体肺炎患儿的MPLI临界值为6.12。MPLI和MP总抗体滴度均随病程升高,MP-DNA则随病程迅速下降;MP-DNA载量较高组的总抗体滴度显著高于MP载量较低组(P<0.05)。病程2周内抗体阴性组的MPLI显著大于抗体阳性组(P<0.05)。结论 MPLI提供了标准化的MP-DNA定量值,对肺炎支原体感染的早期诊断有重要价值。
ObjectiveTo study the role of Mycoplasma pneumoniae （MP） load and antibody measurements in the diagnosis of MP pneumonia.MethodsA total of 115 children with MP pneumonia and 400 healthy children were enrolled. The MP load and total antibody level were measured at different stages, and the MP load index （MPLI） was calculated.ResultsThe cut-off value of MPLI for MP infection was 6.12. MPLI and total antibody titer increased during the course of the disease, while MP-DNA decreased rapidly. Within the same time of blood collection, the group with a higher MP load had a signiifcantly higher total antibody titer than the group with a lower MP load （P〈0.05）. Within 2 weeks of the course of the disease, the negative antibody group had a significantly higher MPLI than the positive antibody group （P〈0.05）.ConclusionsMPLI provides a standardized quantitative value of MP-DNA and plays an important role in the early diagnosis of MP infection.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2016年第10期984-987,共4页
Chinese Journal of Contemporary Pediatrics
基金
厦门市科技局计划项目(3502Z20144048)
关键词
肺炎支原体
肺炎支原体载量指数
总抗体滴度
儿童
Mycoplasma pneumoniae
Mycoplasma pneumoniae load index
Total antibody titer
Child
