摘要
目的:探讨重楼皂苷Ⅶ(Paris saponin Ⅶ,PSⅦ)对人结肠癌SW480细胞凋亡和周期的影响及其机制。方法:采用Cell Counting Kit-8(CCk-8)试剂盒方法观察PSⅦ对SW-480细胞增殖的影响;流失细胞术研究PSⅦ对SW480细胞凋亡和周期的作用;培养SW-480细胞并给予1.0μmol/L、2.0μmol/L和4.0μmol/L PSⅦ 24 h后,采用Western blot法检测凋亡相关蛋白Caspase-3、Caspase-8、Caspase-9、Bax和Bcl-2及周期相关蛋白Cdk-4、Cdk-6、Cyclin D1和p21的表达变化。结果:PSⅦ可明显抑制SW-480细胞增殖,并诱导其凋亡,其IC50值为5.25±0.46μmol/L;PSⅦ可促进SW-480细胞Caspase-3、Caspase-8、Caspase-9、Bax及p21的表达,降低Bcl-2、Cdk-4、Cdk-6和Cyclin D1的表达。结论:PSⅦ可以通过线粒体和死亡受体途径诱导SW-480细胞凋亡;将SW-480细胞周期阻滞于G1期是通过上调p21,抑制Cdk-4、Cdk-6与Cyclin D1周期调控蛋白的表达。
Objective： To investigate the effects of Paris saponin VII （PSVII） on the apoptosis and the cell cycle of human colon cancer SW-480 cells and the potential mechanisms. Methods： The effects of different doses of PSVII on the proliferation of SW-480 cells were observed by Cell Counting Kit-8 （CCk-8）assay, and the effects of PSVII on the apoptosis and the cell cycle of SW-480 cells were investigated by flow cytometric analysis. After treated with different concentration （1.0, 2.0, 4.0 p^mol/L） of PSVII, Western Blot was used to test the expressions of Caspase-3, Caspase-8, Caspase-9, Bax and Bcl-2, and the cycle associated protein Cdk-4, Cdk-6, Cyclin D1 and p21 in SW-480 cells. Results： PSVII could obviously inhibit the proliferation of SW-480 cells significantly in a dose dependent manner. PSVII could induce the apoptosis of SW-480 cells and the IC^0 was 5.25 ＋ 0.46 txmol/L. PSVII could induce the apoptosis of SW-480 cells by increasing the expression of Caspase-3, Caspase-8, Caspase-9 and Bax, while decreasing the expression of Bcl-2 in SW-480 cells. PSVII could reduce the expression of Cdk-4, Cdk-6 and Cyclin D1, while increased the expression of p21 in SW-480 cells. Conclusion： PSVII can induce the apoptosis of SW-480 cells through both the death receptor pathway and mitochondrion pathway. PSVII can arrest the cell Cyclein G1 phase by increasing the expression of p21, while decreasing the expression of Cdk-4, Cdk-6 and Cyclin D1 in SW-480 cells.
出处
《现代生物医学进展》
CAS
2016年第30期5809-5813,共5页
Progress in Modern Biomedicine
基金
陕西省社会发展科技攻关项目(2015SF299)
