摘要
目的观察莪术醇与奥沙利铂联合肿瘤内注射对裸鼠胃癌移植瘤生长、瘤细胞增殖的影响,并探讨其机制。方法 20只裸鼠,建立胃癌移植瘤模型,将其随机分为对照组、莪术醇组、奥沙利铂组、联合组各5只,对照组肿瘤内注射生理盐水(0.05 m L/10 g),莪术醇组肿瘤内注射莪术醇(100 mg/kg),奥沙利铂组肿瘤内注射奥沙利铂(30 mg/kg),联合组肿瘤内注射莪术醇(100 mg/kg)与奥沙利铂(30 mg/kg),每4 d 1次,共4次。观察各组肿瘤生长情况,MTT法测算各组肿瘤组织中的瘤细胞存活率,荧光定量PCR检测各组肿瘤组织Bcl-2、Caspase-3 mRNA,免疫组化法检测各组肿瘤组织Bcl-2、Caspase-3蛋白。结果与对照组相比,联合组、莪术醇组、奥沙利铂组肿瘤体积小、重量低、瘤细胞存活率低、Caspase-3表达高、Bcl-2表达低,且联合组变化更明显(P均<0.05)。结论莪术醇联合奥沙利铂肿瘤内注射可抑制裸鼠胃癌移植瘤生长并抑制瘤细胞增殖,这可能与上调Caspase-3、下调Bcl-2表达有关。
Objective To observe the effects of curcumol combined with oxaliplatin on xenograft growth of gastric cancer cells in nude mice,then to explore the mechanism. Methods Twenty nude mice were selected to establish the xenograft models of gastric cancer cells in nude mice. Nude-mice xenograft models were randomly divided into four groups（ n= 5 in each group） ： the control group,curcumol group,oxaliplatin group and curcumol combined with oxaliplatin group（ combined treatment group）,then they were respectively treated with normal saline（ 0. 05 m L/10 g）,curcumol（ 100 mg/kg）,oxaliplatin（ 30 mg/kg） and curcumol（ 100 mg/kg） combined with oxaliplatin（ 30 mg/kg）,once every 4 days for four times. The growth of tumor was recorded. The cell survival rate of tumor cells in cancer tissues was determined by MTT. The expression levels of Caspase-3,Bcl-2 mRNA and protein were detected by fluorescence quantitative RT-PCR and immunohistochemistry. Results Compared with the control group,the tumor volume,weight,cell survival rate and the expression of Bcl-2 was decreased but the expression of Caspase-3 was increased in the curcumol group,oxaliplatin group and combined treatment group,and these changes were more significant in the combined treatment group（ all P〈0. 05）.Conclusion The curcumol combined with oxaliplatin may inhibit the xenograft growth and proliferation of gastric cancer tumor cells in nude mice,and the mechanism may be related to the up-regulation of Caspase-3 and down-regulation of Bcl-2.
出处
《山东医药》
CAS
北大核心
2016年第35期21-23,共3页
Shandong Medical Journal
基金
国家自然基金面上项目(81372580)
河北省中医药管理局科研基金资助项目(2014145D)
