摘要
目的:探讨糖尿病模型大鼠的痛反应、痛感觉、痛情绪的变化及其心理生理特性。方法:成年SD大鼠18只随机均分为对照组和糖尿病模型组,模型组用链唑霉素(STZ)腹腔注射法制备。在造模前、造模后第21天,检测两组大鼠的光辐射热甩尾反应潜伏期。并在第21天的检测后,对麻醉大鼠的痛感觉代表区异颗粒区(DZ)和痛情绪代表核团基底外侧杏仁核(BLA)电活动,以及心电、呼吸肌肌电、皮肤电导、体温进行心理生理学记录,并观察热、夹尾刺激的影响。结果:模型组大鼠血糖在STZ注射后维持在>16 mmol/L,其甩尾反应潜伏期较正常组缩短(P<0.01)。模型组大鼠的BLA基础放电频率高于正常组(P<0.01),两组大鼠BLA、DZ的放电频率在两种伤害性刺激时均提高(P<0.05),热刺激时,模型组大鼠反应时BLA、DZ的放电频率增加高于正常组(P<0.05),而在夹尾刺激时,模型组仅BLA的放电频率增加高于正常组(P<0.05)。同时,两种刺激导致两组大鼠均出现心率、呼吸频率加快,但正常组在热刺激后心率、呼吸频率较快恢复,模型组未完全恢复(P<0.01)。结论:糖尿病模型大鼠的痛反应显著敏化,且伤害性刺激所诱发的痛感觉、痛情绪及其生理反应亦明显增强。
Objective： To investigate the underlying psychophysiological mechanisms of diabetes-induced pain in rat models via observing the changes of pain response,sensation and emotion in model rats. Methods： Eighteen adult male rats were randomized into control group and diabetic model group. Rat models were induced by intraperitneal injection of streptozotocin（ STZ）,and light radiant heat test was performed to measure the tail-flick latency of rats in the two groups before and on day 21 after STZ induction. Electrical activities at dysgranular zone（ DZ）,the pain sensation-related brain region,and basolateral amygdala（ BLA）,the pain emotion-related nucleus were both extracellularly recorded with simultaneous observation of physiological functions,including electrocardiograph,electromyography of respiratory muscles,skin conductance and body temperature,in anaesthetized rats. Thermal stimulation and tail pinch stimulation were used to elicit nociceptive responses. Results： The model rats had blood glucose concentration over 16 mmol / L after injection of STZ,significantly shortened tail-flick latency（ P〈0.01）,and higher BLA basal firing rate（ P〈0.01）. Although the firing rate of BLA and DZ was increased in both groups by the two types of stimulation（ P〈0.05）,yet rats in model group had increased firing rate at BLA and DZ to the radiant test（ P 〈0.05）,and exclusively increased BLA firing rate to the tail pinch（ P〈0.05）. The heart rate and the respiratory rate were increased in both groups by two types of stimulation,and recovery of the heart and respiratory rate was quicker following stimulation in the control group,whereas was incomplete in model group（ P〈0.01）. Conclusion： The pain response appears more sensitized for diabetic rats that have significantly enhanced pain sensation,pain emotion and physiological functions under nociceptive stimulations.
出处
《皖南医学院学报》
CAS
2016年第5期409-413,417,共6页
Journal of Wannan Medical College
基金
安徽省大学生创新创业训练计划项目(AH201410368062)
人文与管理学院大学生科研培育基金项目(RG201430)
国家自然科学基金项目(31271155)
关键词
糖尿病神经病理性痛
痛反应
痛觉
痛情绪
心理生理学
diabetic neuropathic pain
pain response
pain sensation
pain emotion
psychophysiology
