摘要
目的:探讨针刺"百会"透"曲鬓"穴拮抗急性脑出血大鼠炎性损伤的作用机制。方法:54只Wistar大鼠随机分为假手术组、模型组及针刺组,每组按造模后1、3、7d分为3个亚组,每组6只。采用自体血注入法建立脑出血大鼠模型。针刺组取"百会"透患侧"曲鬓"进行治疗,24h治疗1次。采用Longa评分法及肢体对称实验评分法对脑出血后大鼠神经功能进行评估,采用免疫组化法检测脑出血组织Toll样受体4(TLR-4)、肿瘤坏死因子(TNF-α)、白介素(IL)-6的阳性表达。结果:模型组大鼠脑出血损伤后出现不同程度的神经功能缺损症状表现,术后3d大鼠神经功能缺损最重;针刺后神经功能缺损明显减轻,针刺组各时间点与模型组比较差异有统计学意义(P<0.01)。在各时间点假手术组大鼠脑组织可见少量TNF-α、TLR-4、IL-6阳性表达;模型组在各时间点TNF-α、TLR-4、IL-6阳性表达均高于假手术组(P<0.01);针刺组在各时间点与模型组比较,TNF-α、TLR-4、IL-6阳性表达明显下调(P<0.01)。TLR-4与IL-6表达呈正相关,TLR-4与TNF-α表达呈正相关。结论:针刺"百会"透"曲鬓"穴可以抑制TLR-4蛋白的表达,降低血肿组织中炎性因子TNF-α、IL-6的含量,减轻脑出血后炎性损伤,改善大鼠神经功能缺损表现。
Objective To observe the effect of penetrative needling of＂Baihui＂（GV 20）to＂Qubin＂（GB 7）on neurologic functions and expression of tumor necrosis factor（TNF）-α,interleukin（IL）-6and toll-like receptor 4（TLR-4,involving in inflammatory reactions）in the tissue around the local cerebral hematoma in rats with intracerebral hemorrhage（ICH）,so as to provide evidence for clinical treatment of ICH.Methods Fifty-four male Wistar rats were randomly divided into sham control,model and acupuncture groups,and then further divided into three time-point subgroups（1,3,7days after modeling,n=6/subgroup）.The ICH model was established by injection of the rat＇s autoblood（50μL）into the putaman region（P：0.2mm,R：3.5mm）in a stereotaxic apparatus and confirmed by Berderson＇s neurologic examination grading system（0-3points）.The neurologic function was assessed by using Longa＇s scoring（5-points）and footfault asymmetry testing[footfault index=（contra faultsipsi faults）/total steps in 2min].For penetrative needling,an acupuncture needle was inserted into GV 20 and controlled to advance to GB 7on the affected side and retained for 30 min,once daily.The expression of TNF-α,IL-6and TLR-4in the cerebral tissue around the putaman was detected by immunohistochemistry.Results After penetrative needling stimulation,the increased Longa＇s score and footfault asymmetry score in ICH rats were significantly decreased on day 1,3 and 7 after modeling（P0.01）,suggesting an improvement of neurologic function after the treatment.Immunohistochemical staining outcomes of the cerebral tissue surrounding the autoblood injection site showed that the expression levels of TNF-α,IL-6and TLR-4proteins on day1,3and 7were considerably higher in the model group than in the control group（P0.01）,and markedly lower in the acupuncture group than in the model group（P0.01）,suggesting a suppression of the proinflammatory factors and TLR-4levels around the locus of the brain after needling intervention.A positive correlation existed between the expression levels of TLR-4and IL-6/TNF-α.Conclusion Penetrative needling stimulation of GV 20 to GB 7can reduce the levels of proinflammatory factors TNF-αand IL-6,and TLR-4in the ICH tissues in rats with cerebral hemorrhage,which may contribute to its effect in improving neurological function.
作者
陈秋欣
邹伟
孙晓伟
于学平
戴晓红
牛明明
滕伟
包宇
于薇薇
马慧慧
CHEN Qiu-xin ZOU Wei SUN Xiao wei YU Xue-ping DAI Xiao-hong NIU Ming-ming TENG Wei BAO Yu YU Wei-wei MA Hui-hui(The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, China 1.Heilongjiang University of Chinese Medicine, Harbin 15004)
出处
《针刺研究》
CAS
CSCD
北大核心
2016年第5期410-416,共7页
Acupuncture Research
基金
国家自然科学基金(No.81473764
No.81273824)
黑龙江省自然科学基金重点项目(No.ZD 201204)
教育部博士点基金项目(No.20102327110003)
黑龙江中医药大学博士创新基金项目(No.2015bs 03)
关键词
针刺(透刺)
脑出血
肿瘤坏死因子-α
白介素-6
TOLL样受体4
Acupuncture（penetrative needling）
Cerebral hemorrhage
Tumor necrosis factor-α
Interleukin-6
Toll-like receptor-4
