摘要
目的基于主成分分析(PCA)和多指标综合指数法,评价丹参不同有效部位丹参提取物(DT)、丹参总酚酸提取物(DF)、丹参总酮提取物(DST)、丹参总酚酸合用总酮提取物(DF+DST)对急性血瘀大鼠血液流变学和凝血功能的影响。方法采用sc盐酸肾上腺素和冰水浴共同刺激复制大鼠急性血瘀模型,通过测定全血黏度(WBV)、血浆黏度(PV)、血沉(ESR)和红细胞压积(HCT),观察丹参不同有效部位不同剂量对血瘀大鼠血液流变学的影响;通过测定凝血酶时间(TT)、凝血酶原时间(PT)、活化部分凝血酶时间(APTT)、血浆纤维蛋白原(FIB)量及血小板最大聚集率(ADP),观察丹参不同有效部位对血瘀大鼠凝血功能及血小板聚集的影响。采用PCA法和多指标综合指数法综合评价丹参不同有效部位的总活血化瘀效应。结果与对照组比较,模型组大鼠血液流变学和凝血功能指标均有显著性差异;与模型组比较,丹参不同部位低、中、高3个剂量给药组均能较好地改善血瘀大鼠的血液流变学和凝血功能指标,并呈一定的量效关系。PCA和多指标综合指数法综合评价得出,丹参不同有效部位不同剂量组中DF+DST高剂量组的活血化瘀作用最好;相同剂量下,各剂量下的DF+DST给药组活血化瘀效应较好。结论丹参不同有效部位能明显改善血瘀大鼠的血液流变学及凝血功能异常,丹参总酚酸和丹参总酮2个有效部位配伍活血化瘀效果优于各有效部位单用的效果。
Objective To evaluate the activating blood circulation and dissipating blood stasis effects of the compatibility between two effective parts（DT, DF, DST, and DF ＋ DST） of Salvia miltiorrhiza. with different doses on rats with acute blood stasis. Methods Acute blood stasis rat model was induced by sc injection of adrenaline and ice water bath. The blood stasis mice were ig administered with different effective parts of S. miltiorrhiza. The whole blood viscosity（WBV）, plasma viscosity（PV）, erythrocyte sedimentation rate（ESR）, and haematocrit（HCT） were determined to observe the effects of two effective parts of Danshen on hemorheology of blood stasis rats. And the thrombin time（TT）, prothrombin time（PT）, activated partial thromboplastin time（APTT）, and plasma fibrinogen（FIB） were tested to observe the effects of different effective parts of S. miltiorrhiza on blood coagulation function of blood stasis rats. In addition, the maximum aggregation induced by adenosine diphosphate（ADP） was tested to observe the effect of different active parts of S. miltiorrhiza on platelet aggregation index of blood stasis rats. Then principal component analysis（PCA） and multi-attribute comprehensive index methods were both used to comprehensively evaluate the total activating blood circulation and dissipating blood stasis effects of different effective parts of S. miltiorrhiza. Results The hemorheological indexes and coagulation parameters of model group had the significant differences with normal group. Compared with model group, the effective parts of S. miltiorrhiza with different doses could improve the blood hemorheological indexes and coagulation parameters in acute blood stasis rats with dose-effect relation. Based on the PCA and multi-attribute comprehensive index methods, the group of high-dose DF ＋ DST had the best effect of activating blood circulation and dissipating blood stasis effect in all medication administration teams. Under the condition of same dose of the effective parts of S. miltiorrhiza, the DF ＋ DST could obviously improve the hemorheology and blood coagulation function in acute blood stasis rats. Conclusion The combination of DF and DST has the better effects of activating blood circulation and dissipating blood stasis in comparison with those of single part. It could provide the scientific basis for more effective application of the compatibility between salvianolic acids and tanshinones in modern clinic medicine.
出处
《中草药》
CAS
CSCD
北大核心
2016年第18期3239-3247,共9页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81274058
81573714)
江苏高校优势学科中药学学科建设工程资助
关键词
丹参
有效部位
配伍
活血化瘀
主成分分析
多指标综合指数法
急性血瘀
Salvia miltiorrhiza Bge
effective parts
compatibility
activating blood circulation and dissipating blood stasis
principal component analysis
multi-attribute comprehensive index method
acute blood stasis
