摘要
目的研究α-半乳糖苷酶对人体肝脏血型抗原的清除效果，探讨肝脏血型转换的可行性。方法采用肝移植手术中B血型病肝建立离体肝脏的灌注模型。用UW液＋／-α-半乳糖苷酶灌注离体肝脏，免疫荧光分析酶对肝脏B抗原的清除效果。结果用含α-半乳糖苷酶的uw液灌注离体肝脏，免疫组化显示，低温灌注保存后的肝脏血型抗原的含量明显降低。灌注后1ht3抗原减少至灌注前平均水平的58％、2h为10％、4h为4％。不同时间血型抗原水平差异有统计学意义（P〈0．05）。对照组血型抗原水平未见明显的变化。结论α-半乳糖苷酶可以有效清除人的离体肝组织B抗原。虽然并未达到不可测水平，但可基本实现人体器官肝脏B→O的血型转换，对研究人体器官的血型转换有重要意义。
Objective To study blood type B antigen elimination with c^-galactosidase in human liv- er tissue, and discuss the feasibility of blood type conversion in human liver. Methods The liver specimens from patients with blood type B in liver transplantation were collected, and an in vitro liver peffusion model was established. The in vitro livers were perfused with UW solution ＋ / - α-galactosidase. The effect of en- zyme in B antigen of human liver were analyzed by immunofluorescence. Results With UW solution contai- ning α-galactosidase to perfuse the in vitro livers, immunohistochemistry showed the level of blood type B an- tigen in liver was significantly reduced after hypothermic perfusion and preservation. The B antigen level in 1 h perfusion was reduced to approximate 58% of this figure prior to perfusion, in 2 h was 10% , and in 4 h was 4%. Among the different intervals, the blood group antigen levels showed significant differences （P 〈 0. 05）. In the control group, the blood group antigen levels showed no obvious change on statistical analysis. Conclusions α-galactosidase was effective to clear blood type B antigen in isolated liver tissue. In the ex- perimental group, Although the B antigen did not fall to a undetectable level, liver blood type conversion from B→O remains a promising potential which has been meaningful for related researches on blood type conversion of human organs.
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2016年第10期677-681,共5页
Chinese Journal of Hepatobiliary Surgery
基金
天津市卫生局科技基金（2010KR03）
关键词
Α-半乳糖苷酶
人体
肝脏
血型转换
α-galactosidase
Human body
Liver
Blood type conversion
