摘要
以盆栽大豆北豆44为实验材料,研究在干旱胁迫下外源脱落酸(ABA)和油菜素内酯(BR)对大豆抗旱性的影响。结果表明,在正常供水条件下,BR对光合有抑制作用,ABA促进气孔关闭,降低胞间CO_2浓度,BR加强了ABA的作用。在干旱胁迫条件下,高浓度的BR(0.4mg/L)加剧了干旱对大豆幼苗叶片的伤害,但ABA可以缓解其伤害程度;低浓度的BR促进叶绿素的增加,ABA可缓和高浓度BR对叶绿素的抑制作用;BR(0.2、0.4mg/L)可以缓解干旱对大豆光合的影响,ABA和BR共同作用对大豆光合作用具有更好的效果;BR降低了ABA促进气孔关闭的作用,使得PSⅡ反应中心Fv/Fm和Ф_(PSII)升高,与净光合速率的变化相一致。因此ABA(2mg/L)和BR(0.2、0.4mg/L)共同作用对大豆有更好的抗旱作用。因此,干旱胁迫下,喷施一定浓度的脱落酸和油菜素内酯可有效缓解干旱胁迫影响,维持大豆正常生理代谢。
Soybean cultivar Beidou 44 seedlings were used as test materials in pod experiment to study the effects of exogenous abscisic acid（ABA） and brassinolide（BR） on drought resistance of soybean. The results showed that with normal water supply,BR inhibited the photosynthesis of seedling,ABA promoted stomatal closure,and decreased intercellular CO2 concentration,BR enhanced the effect of ABA. Under drought stress,high concentration of BR（0. 4mg/L） accelerated the damage of seedling caused by drought,but ABA relieved the damage severity; low concentration of BR increated chlorophyll content,ABA could alleviate the effect of BR on chlorophyll; BR（0. 2,0. 4mg/L） reduced the influence on soybean photosynthesis under drought. The combination of ABA and BR showed greater effect on photosynthesis in soybean; BR reduced the promotional effect of stomatal closure caused by ABA,and increased Fv/Fm and ФPSIIin PSII reaction center,which were consistent with the effect on photosynthesis. Therefore the interaction of ABA（2 mg/L） and BR（0. 2 and 0. 4 mg/L） made greater contribution to drought resistance of soybean. In conclusion,appropriate concentration of ABA and BR could effectively alleviate the drought stress,and maintain the normal metabolism of soybean.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2016年第5期605-610,共6页
Chinese Journal of Oil Crop Sciences
基金
哈尔滨市优秀学科带头人项目(2012RFXXN085)
关键词
干旱胁迫
大豆
外源激素
脱落酸
油菜素内酯
Soybean
Drought stress
Exogenous hormone
Abscisic acid
Brassinolide
