摘要
目的：建立同时测定益肤丸中香豆素类化合物含量的方法。方法：采用高效液相色谱法。色谱柱为Inertsil ODS-3,流动相为乙腈-水（49∶51,V/V）,流速为1.0 ml/min,检测波长为320 nm,柱温为30℃,进样量为20μl。结果：蛇床子素、异欧前胡素和二氢欧山芹醇当归酸酯检测质量浓度线性范围分别为5.16-206.4μg/ml（r=0.999 9）、37.40-1 495.89μg/ml（r=0.999 9）、9.95-318.34μg/ml（r=0.999 9）;精密度、稳定性、重复性试验的RSD〈3.0%;加样回收率分别为96.05%-103.19%（RSD=2.18%,n=9）、96.90%-103.09%（RSD=2.07%,n=9）、95.50%-103.57%（RSD=2.15%,n=9）。结论：该方法操作简便,精密度、稳定性、重复性较好,可用于益肤丸中香豆素类化合物含量的同时测定。
OBJECTIVE：To establish a method for the simultaneous determination of coumarin compounds in Yifu pill. METHODS：HPLC was performed on the column of Inertsil ODS-3 with mobile phase of acetonitrile-water（49 ∶ 51,V/V）at a flow rate was 1.0 ml/min,the detection wavelength was 320 nm,column temperature was 30 ℃,injection volume was 20 μl. RESULTS：The linear range was 5.16-206.4 μg/ml for osthole（r =0.999 9）,37.40-1 495.89 μg/ml for isoimperatorin（r =0.999 9） and 9.95-318.34 μg/ml for columbianadin（r=0.999 9）;RSDs of precision,stability and reproducibility tests were lower than 3.0%;recoveries were 96.05%-103.19%（RSD=2.18%,n=9） for osthole,96.90%-103.09%（RSD=2.07%,n=9） for isoimperatorin,95.50%-103.57%（RSD=2.15%,n=9）for columbianadin. CONCLUSIONS：The method is simple with good precision,stability and reproducibility,and can be used for the simultaneous determination of coumarin compounds in Yifu pill.
出处
《中国药房》
CAS
北大核心
2016年第30期4299-4301,共3页
China Pharmacy
