摘要
目的:研究牙髓炎不同阶段P2X受体家族在大鼠脑干中的表达变化。方法::本实验在大鼠左上颌第一磨牙颌面开髓,并暴露于口腔菌群环境,诱导牙髓炎症。取牙髓炎症第1天、第3天、第7天和第28天时,大鼠双侧脑干的三叉神经脊束核尾端亚核(Vc)和丘脑的腹后中核(VPM),采用免疫组化和免疫荧光方法观察P2X受体家族(P2X1-7受体)的表达模式。结果:实验观察到牙髓炎症第1、3、7天显示急性牙髓炎症的组织学表现,其中第3天最为典型,而第28天显示牙髓坏死和慢性根尖周炎的组织学表现;P2X1-6受体在Vc和VPM中均表达在神经元的胞浆中,而P2X7表达在Vc和VPM中在小胶质细胞中;P2X2,P2X4和P2X7受体在急性牙髓炎症期在同侧Vc中的表达增加;P2X1-5和P2X7的表达都在双侧VPM中均上升,开髓3d时,对侧VPM中P2X1-5和P2X7的平均表达面积显著高于同侧。结论:除P2X6受体持续高表达外,P2X1-5和P2X7受体均可受到牙髓炎症的诱导,在Vc和VPM中表达增高。
Objective：To study the expression of P2 Xreceptors in the trigeminal spinal subnucleus caudalis（Vc）and the ventral posteromedial nucleus（VPM）induced by dental pulp inflammation.Methods：Cavities were prepared on the left first upper molars via a 1/2 # round ball drill,and the cavities with exposed dental pulp were left open to the oral microflora for 1,3,7and 28 days.The Vc and VPM were harvested at specific time intervals and underwent further immunofluorescent and immunohistochemical procedures to assess the expression patterns of P2X1-7.Results：Acute inflammation was induced by dental pulp exposure from 1dto 7d,and chronic inflammation around the apical area of the root was observed after 28 d.The P2X1-6receptors were expressed in neurons,whereas P2X7 was expressed in microglia.Varying increases in the expression of the P2X1-7receptors were characterized during acute and chronic pulp inflammation.The expressions of P2X2,P2X4 and P2X7in the ipsilateral Vc,and P2X1-5and P2X7 in the bilateral VPM,especially on the contralateral side were increased during acute inflammation process.Conclusion：The present findings suggest that all of the P2 Xreceptors except P2X6 are involved in different ways in the Vc and VPM after dental pulp injury.
作者
陈霞
袁通穗
叶芳
孙瑜
杨健
郭俊
金幼虹
CHEN Xia YUAN Tong- sui YE Fang SUN Yu YANG Jian GUO Jun JIN You -hong.(Department of Oral Medicine Teaching and Research Section, Affiliated Stomatological Hospital, Nanchang University, Nanchang 330006, Chin)
出处
《口腔医学研究》
CAS
CSCD
北大核心
2016年第10期1033-1037,共5页
Journal of Oral Science Research
基金
江西省自然科学基金重点项目(编号:20142BAB205035)
