摘要
The present study was designed to construct the structurally diverse library of tetrahydroprotoberberines(THPBs) by combining the methods of chemical nonselective demethylation and microbial glycosylation. HPLC-MS/MS analyses tentativelyidentified 12 de-methylated and 9 glycosylated derivates of THPBs and 5 rarely oxidized glycosides of THPBs in the library. Through this effort, we achieved not only a variety of the THPBs and their glycosides but also tested the catalytic characteristics and capabilities of G. deliquescens NRRL 1086.
The present study was designed to construct the structurally diverse library of tetrahydroprotoberberines （THPBs） by combining the methods of chemical nonselective demethylation and microbial glycosylation. HPLC-MS/MS analyses tentatively identified 12 de-methylated and 9 glycosylated derivates of THPBs and 5 rarely oxidized glycosides of THPBs in the library. Through this effort, we achieved not only a variety of the THPBs and their glycosides but also tested the catalytic characteristics and capabilities of G deliquescens NRRL 1086.
基金
funded by the National Nature Science Foundation of China(No.21302052)
the Program for New Century Excellent Talents in University
the‘111 Project’from the Ministry of Education of China
the Fundamental Research Funds for the Central Universities(No.JKZ2011017)
