摘要
鉴别藏医临床常用的3种"欧贝"类绿绒蒿药材.采用原植物和药材性状比较,光镜(OM)法观察根、花葶、叶、花的内部组织结构显微特征,扫描电镜(SEM)法观察花粉粒形态以及薄层层析法(TLC)对3种绿绒蒿进行鉴别.结果表明:1)原植物和性状特征可根据花颜色不同或植株大小区分;2)显微组织结构特征可根据根的木质化程度和叶的主脉维管束差异进行鉴别;3)粉末特征以花粉囊内壁细胞的特征最为明显;4)花粉粒表面特征:全缘叶绿绒蒿和红花绿绒蒿的花粉粒为球形或近球形,无萌发孔,全缘叶绿绒蒿花粉粒上的刺状突起较红花绿绒蒿钝,红花绿绒蒿花粉粒表面比全缘叶绿绒蒿平滑;川西绿绒蒿花粉粒类型为3孔沟,表面具刺状雕纹.得出3种绿绒蒿的原植物、性状、内部显微组织结构、粉末特征、花粉粒表面特征等区别明显,可为藏医临床常用的3种"欧贝"类绿绒蒿药材的鉴别提供参考依据.
Objective To identify the common Tibetan medical species of Meconopsis which named oubei.Method The plant morphology and crude drug were observed;microscopic characteristics of root,scape,leaf and flower were observed by optical microscopy;the pollen was observed by scanning electron microscopy,and thin layer chromatography was used to identify M.integrifolia,M.punicea and M.henrici.Result：1）Origin plant and crude drug characteristics could be identified by their flower color.2）Microscopic characteristics could be distinguished by the level of lignifications of root,and difference of vascular.3）Powder characteristics could be identified by the difference of pollen sacs.4）The pollen of M.integrifolia and M.punicea was spherical,nonaperturate,the difference of them was that the spines of M.punicea was more sharper than that of others and the surface of it was much smoother than that of M.integrifolia.The pollen of M.henrici was tricolpate,and the surface with speculate glyphs.And the distinction of microscopic characteristics and TLC of three species of Meconopsis was also obvious.Conclusion The differences of microscopic characteristics of common Tibetan medical species were obvious,which provided basis for their quality evaluation.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第10期8-16,共9页
Journal of Southwest University(Natural Science Edition)
基金
四川省科技计划项目(2014JY10181)
"十三五"国家科技支撑计划项目(2015BAC05B02)
关键词
全缘叶绿绒蒿
红花绿绒蒿
川西绿绒蒿
花粉粒
显微鉴别
Meconopsis integrifolia（Maxim
）Franch
M.punicea Maxim
M.henrici Bur.et Franch
pollen
authentication
