摘要
目的优化藏药砂生槐子总生物碱的纯化工艺。方法盐酸和氨水调节总生物碱水溶液p H值后,HPLC法测定总生物碱(氧化苦参碱、槐果碱、槐定碱、苦参碱)含有量,考察5种大孔树脂(D101、HPD600、H103、NKA-9、LAS-5B)吸附和解吸附行为。结果 H103大孔树脂静态吸附量最大,最佳条件为上样p H值10,上样量5 BV(体积流量15 m L/h),水洗用量8 BV,70%乙醇洗脱用量11 BV(体积流量2 BV/h),4种生物碱平均洗脱率分别为81.54%、88.37%、90.35%、94.28%,平均含有量分别为0.15、0.08、0.09、0.15 mg/g,比优化前分别提高了1.87、20.41、9.72、7.65倍。结论 H103大孔树脂对砂生槐子总生物碱具有较强的吸附能力,富集效果好。
AIM To optimize the purification of total alkaloids from Tibetan medicine Sophora moorcroftiana seeds. METHODS After the p H value of aqueous extract of total alkaloids was adjusted with chloric acid and ammonia water,HPLC was applied to determining the contents of total alkaloids（ matrine,oxymatrine,sophocarpine and sophoridine）. The adsorption and desorption behaviors of five kinds of macroporous resins（ D101,HPD600,H103,NKA-9,LAS-5B） were investigated. RESULTS H103 macroporous resin showed the largest static adsorption capacity. The optimal conditions were determined to be 10 for p H value,5 BV for sample volume at a flow rate of 15 m L / h,8 BV for water consumption,and 11 BV for elution volume of 70% ethanol at a flow rate of 2 BV / h. The average elution rates of four alkaloids were 81. 54%,88. 37%,90. 35% and 94. 28%,whose average contents were 0. 15 mg / g,0. 08 mg / g,0. 09 mg / g and 0. 15 mg / g（ 1. 87,20. 41,9. 72 and 7. 65 times of those before the optimization）,respectively. CONCLUSION H103 macroporous resin holds strong adsorption ability for total alkaloids from Sophora moorcroftiana seeds,therefore can bring forth a good enrichment effect for it.
出处
《中成药》
CAS
CSCD
北大核心
2016年第10期2157-2162,共6页
Chinese Traditional Patent Medicine
基金
青海大学医学院中青年团队项目(2013-KT-1)
青海省科技厅项目(2014-ZJ-716)
关键词
砂生槐子
苦参碱
氧化苦参碱
槐果碱
槐定碱
纯化工艺
大孔树脂
Sophora moorcroftiana seeds
matrine
oxymatrine
sophocarpine
sophoridine
purification
macroporous resins
