摘要
A population of 150 recombination inbred lines （RILs） derived from the cross between rice varieties V20B and CPSLO17, was applied to locate the QTLs related to chalkiness traits and evaluate their genetic effects. A genetic linkage map was constructed based on 8 602 SLAF （specific-locus amplified fragment） markers, combine with the chatkiness traits of the tested lines. Four QTLs that related to chalkiness were detected using MapQTL 5 software, named qC-5a, qC-5b, qC-5c and qC-5d. The LOD threshold values of qC-5a, qC-5b, qC-5c and qC-5d were 4.02, 4.09, 3.94 and 4.1, respectively, explaining 11.6%, 11.8%, 11.2% and 11.8% of the observed phenotypic variance. All the four detected QTL alleles came from Iow-chalkiness parent V20B.
该研究以高垩白度品种V20B和低垩白度品种CPSLO17作为亲本,构建150个V20B/CPSLO17重组自交家系(RIL)为作图群体,进行稻米垩白QTL检测及其遗传效应分析。以垩白度作为衡量稻米垩白高低的表型数据,结合SLAF标签构建的分子连锁图谱,运用Map QTL5软件检测到4个稻米垩白QTL,分别命名为q C-5a、q C-5b、q C-5c和q C-5d。分析结果表明这4个QTL的LOD值分别为4.02、4.09、3.94和4.1,对表型变异的解释率分别为11.6%、11.8%、11.2%和11.8%,且这4个QTL抗性等位基因都来自低垩白亲本CPSLO17。
基金
Supported by Research Institution Program to Serve Enterprises in Guizhou Province(LH[2014]4005)
Science and Technology Research Program of Guizhou Province(G[2012]4010,[2015]5003-3)
Earmarked Fund for Agriculture Research System of Guizhou Province(GZCYTX2015-06)~~
