摘要
利用Smart-RACE的方法,从泰国斗鱼(Betta splendens)脑中克隆得到了神经肽Y(Neuropeptide Y,NPY)的c DNA全长序列.泰国斗鱼NPY基因的c DNA序列为506bp,其中开放读码框300bp,编码99个氨基酸.氨基酸比对分析显示NPY成熟肽的氨基酸序列较保守.通过系统进化树分析,泰国斗鱼NPY与鲈形目鲈鱼等的亲缘关系最近,但鱼类的NPY分子进化分为两个相对独立的分支,显示出独特的特点.利用RT-PCR进行泰国斗鱼雌雄个体的组织分布分析,结果发现泰国斗鱼NPY mRNA的主要在脑中高表达,并显示出明显的组织特异性及性别差异性.本研究为开展NPY分子进化及后续的功能研究提供了研究基础.
The cDNA sequences of Thailand betta（ Betta splendens） neuropeptide Y （NPY） were cloned with the rapid - amplification of cDNA ends （RACE）. The NPY cDNA sequence contains 506 bp nucleotides with the open reading frame （ORF） of 300 bp, encoding a putative protein of 99 amino - acid residues. The sequence alignment shows low similarity in the NPY precursor proteins among vertebrates, but is highly conservative in the NPY mature peptide domains. According to the phylogenetic analysis, the Thailand betta NPY was more closely related to other perciformes fishes, while the molecular evolution of fish NPY develops into two distinct branches. Tissue distribution analysis by RT - PER revealed that the Thailand betta NPY showed the evident tissue - specific profiles in both male and female. The preliminary information is provided by the present study for the molecular evolution and functional study of NPY.
出处
《海南热带海洋学院学报》
2016年第5期11-16,共6页
Journal of Hainan Tropical Ocean University
基金
海南省科技合作专项(KJHZ2015-08)
广东省海洋渔业科技推广专项(A201408A06)
广东海洋大学"海之帆-起航计划"大学生科技创新培育专项(hzfqhjhzrkx2015b15)
关键词
泰国斗鱼
神经肽Y
序列分析
组织分布
betta splendens
neuropeptide Y
sequence analysis
tissue distribution
