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拟南芥KCS12基因的克隆及其对长链脂肪酸代谢调控功能的初步分析

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摘要 本课题从拟南芥中克隆了超长链脂肪酸代谢相关的功能基因At KCS12,转入酵母(INVSC1)表达系统中表达,检测酵母细胞中脂肪酸组成。脂肪酸组成结果分析表明,At KCS12使酵母中棕榈酸(16∶0)和硬脂酸(18∶0)的相对含量显著增加了,十八烯酸(18∶1)的相对含量显著减少了。这些结果共同提示,At KCS12表达的蛋白不能利用酵母中的脂肪酸作为催化底物合成超长链脂肪酸,或者At KCS12蛋白无酶活性。
出处 《南方农业》 2016年第21期183-185,共3页 South China Agriculture
基金 国家自然科学基金项目(项目编号:31260193 31360476 31460213)
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