摘要
目的：建立清毒片中齐墩果酸和熊果酸含量测定的HPLC方法。方法采用HPLC法测定清毒片中齐墩果酸、熊果酸含量，色谱柱为kromasil-C18（250 mm×4.6 mm，5μm），流动相为甲醇-0.5%磷酸水溶液（88∶12），检测波长210 nm，流速1.0 ml/min。结果齐墩果酸和熊果酸的进样量分别在0.06～0.54μg（R＝0.9993）和0.30～2.70μg（R＝0.9997）范围内与峰面积呈良好线性关系；齐墩果酸和熊果酸的加样回收率分别为100.48%和102.21%。结论本方法操作简便、准确、重复性好，可为清毒片的质量控制提供参考。
Objective To establish a method for quality control of oleanolic acid and ursolic acid in Qingdu tablet. Methods The content of oleanolie acid and ursolic acid in Qingdu tablet were determined by high performance liquid chroma-tography（HPLC）. The analytical column was kromasil-C18 （250 mm × 4.6 mm, 5 μm）. The mobile phase consisted of methanol-0.5% phosphoric acid solution （88：12）. The UV detection wavelenghth was 210 nm. The flow rate was 1.0 ml/min. Results Oleanolic acid and ursolic acid showed a good linearity in range of 0.06-0.54μg （R=0.999 3） and 0.30-2.70μg （R=0.999 7）. The average recovery rates of Oleanolic acid and ursolic acid were 100.48% and 102.21%, respectively. Conclusions This method is simple, rapid and accurate. It could be used for the quality control and quality study of the Qingdu tablet.
出处
《国际中医中药杂志》
2016年第10期932-935,共4页
International Journal of Traditional Chinese Medicine
关键词
清毒片
白花蛇舌草
齐墩果酸
熊果酸
高效液相色谱
质量控制
Qingdu tablet
Hedyotis diffusa
Oleanolic acid
Ursolic acid
High performance liquid chromatography
Quality control
