摘要
为了探讨菌渣对黑麦草(Lolium perenne)根际土壤微生物群落结构的影响,测定了添加不同量菌渣后黑麦草根际土壤的磷脂脂肪酸(phospholipid fatty acids,PLFAs),比较分析了不同处理下黑麦草根际土壤微生物PLFAs组成、相对含量和微生物群落结构的差异。结果表明,施用菌渣后,黑麦草植株根际土壤微生物磷脂脂肪酸生物标记种类和总量均高于对照组。进一步分析表明,菌渣处理可以改变黑麦草根际土壤微生物种群结构,有利于根际土壤中细菌、真菌和放线菌的生长。同时,采用菌渣处理黑麦草后,其根际土壤微生物群落的Simpson、Shannon-Wiener和Pielou指数均得以提高,表明菌渣可以增加黑麦草根际土壤中微生物群落多样性,推测其可以改善根际土壤质量。
The present study dealt with the effect of the edible fungus residue on the ecological characteristics of microorganism fatty acids in the rhizosphere of ryegrass.The phospholipid fatty acids(PLFAs)of the soil samples were detected by GC of Agilent 6890 N.Pattern,content and structure of the microbial PLFAs in ryegrass rhizosphere were evaluated.The results showed that the types and content of microbial PLFAs in the rhizosphere of ryegrass adding edible fungus residue were higher than those in the control.In further,the microbial population structure could be changed due to improving the growth of bacteria,fungi and actinomycetes by the treatments with adding edible fungus residue.The edible fungus residue could increase the microbial community diversity in the soil,in which,the enhancement of Shannon-Wiener index of community dominance,Simpson index of community abundance and Pielou index of uniformity was revealed.It was guessed that the quality of rhizosphere soil could be improved by adding edible fungus residue.
出处
《草业科学》
CAS
CSCD
北大核心
2016年第10期1942-1950,共9页
Pratacultural Science
基金
国家自然基金项目(31370059)
福建省自然基金项目(2014J06012)
福建省省属公益类科研院所基本科研专项(2014R1018-13)
福建省农业科学院青年英才计划项目(YC2015-7)
中国科技部科技支撑计划项目(2012BAD14B15)
国家公益性行业(农业)科研专项(201303094)
关键词
根际土壤
磷脂脂肪酸
群落结构
微生物多样性
rhizosphere
phospholipid fatty acids
community structure
microbial diversity