摘要
目的:探讨丹参水提液对小鼠精子质量的影响。方法:选择40只成年雄性昆明小白鼠,随机分为低浓度组、中浓度组、高浓度组、空白对照组、阳性对照组5组,每组8只;低、中、高浓度组小鼠每天分别灌胃1 500、3 000、6 000 mg/kg丹参水提液,空白对照组小鼠每日灌胃10 m L/kg生理盐水,阳性对照组小鼠一次性注射环磷酰胺100 mg/kg;30 d后处死小鼠,并检测各组小鼠的体重变化、脏器系数及精子质量。结果:(1)低、中、高浓度组小鼠每周体重、体重增长量、睾丸系数及附睾系数与空白对照组比较差异均无统计学意义(P>0.05),但各组体重及增量均显著高于阳性对照组(P<0.05)。(2)与空白组比较,低、中、高浓度组精子浓度、畸形率、存活率、总活力、PR、VAP、VSL均有改善,且高浓度组有统计学差异(P<0.05),各组精子质量均显著优于阳性对照组(P<0.05)。结论:丹参水提液能够明显改善小鼠精子的存活、形态及运动能力。
Objective To research effects of water extraction of Salvia miltiorrhiza in sperm quality of mice. Methods Forty adult male Kunming mice were randomly divided into 5 groups, including low concentration group, medium concentration group, high concentration group, control group and positive control group in eight mice in each group. The low, medium and high concentration groups were given by gavage with1 500, 3 000 and 6 000 mg / kg water extraction of salvia miltiorrhiza once a day respectively, while the control group was given gavage with 10 m L / kg normal saline daily, as well as the positive control group was injected with 100 mg / kg cyclophosphamide once; All mice were sacrificed after 30 days. We measured the weight gain,viscera coefficient and sperm quality in each mouse. Results There were no statistical difference in the weight of every week, increase of the weight, testis and epididymis coefficients among the control group, the low,medium and high concentration groups（P〉0.05）, while the weight and weight increase were significantly higher than that in the positive control group（P〈0.05）. Sperm concentration, viability, morphology, motility,PR, VSL, VAP of low, medium and high concentrations group were significantly improved than the control group（P〈0.05）, and the sperm quality in the four groups were higher than that in the positive control group（P〈0.05）. Conclusion Water extraction of salvia miltiorrhiza can obviously improve the sperm viability,morphology, and motility of mice.
出处
《实用医学杂志》
CAS
北大核心
2016年第20期3342-3345,共4页
The Journal of Practical Medicine
基金
广东省中医药局基金资助项目(编号:20151247)
关键词
丹参水提液
精子
存活率
活力
形态
Water extraction of Salvia miltiorrhiza
Sperm
Viability
Motility
Morphology
