摘要
目的观察应用种植体联合骨组织工程技术,修复犬下颌骨节段缺损的效果。方法体外扩增培养、成骨诱导犬BMSCs。将第2代细胞复合珊瑚后修复犬自体右侧下颌骨3 cm的节段缺损,术后32周植入种植体(实验组n=3);同时,以邻近正常骨植入种植体作为对照(n=3)。植入4周、12周、26周后,分别通过影像学、大体形态观察、组织学和生物力学等方法,检测骨缺损的修复效果。结果植入后26周,X线片和CT均显示种植体与实验组及对照组骨质为良好骨性愈合,实验组种植体周围新生骨密度较高。Micro-CT显示,实验组骨密度和对照组间无显著性差别(P>0.05)。大体观察见种植体与组织工程骨和正常骨均形成紧密连接。组织学显示,实验组与对照组均有较多成熟骨结构。生物力学测试结果表明,实验组与正常下颌骨力学强度无显著性差异(P>0.05)。结论自体成骨诱导BMSCs复合珊瑚形成的组织工程化骨,可较好地修复犬下颌骨节段缺损,植入种植体后可进一步促进骨成熟。
Objective To investigate the effect of repairing canine segmental mandibular bone defects by using implant combining with bone tissue engineering. Methods A 3 cm segmental mandibular defect was created, and the defect was repaired with cell-scaffold constructs formed by in vitro expanded and osteogenically induced BMSCs （P2） and coral. Implants were embedded into the mandible 32 weeks post-operation （n=3）; the same was disposed for the adjacent mandible as control （n=3）. Imaging, gross view, histological and biomechanical methods were taken to detect the repairing effects of the bone defect at 4 weeks, 12 weeks, 26 weeks respectively after implantation. Results A well implant-osseous healing was identified in both experimental group and control group by X-ray and computed tomography （CT） 26 weeks after implantation, while the experimental group showed a higher neogenetic bone density around the implant. Micro-CT indicated no significant density difference between the experimental group and the control group （P〉0.05）. Closely connection were formed between the implant and the normal bone as well as the tissue engineered bone. Histologically, lots of mature bone trabecula was showed in both groups, No significant mechanical-strength difference was found in mandible between the biomeehanical testing group and the normal group （P〉0.05）. Conclusion The tissue-engineered bone formed by autologous osteogenic induction of BMSCs and coral carl be a favorable remediation of the canine mandible. Additionally, the implanted implant can further promote bone maturation.
出处
《组织工程与重建外科杂志》
2016年第5期285-288,共4页
Journal of Tissue Engineering and Reconstructive Surgery
基金
国家自然科学基金(81471886)
上海市科委重点项目(134119a2000)
关键词
种植体
下颌骨修复
骨髓基质干细胞
珊瑚
Implant
Mandibular reparation
Bone marrow stromal cells
Coral
