摘要
目的探讨中性粒细胞明胶酶相关载脂蛋白(NGAL)在脑出血(ICH)后继发性脑损伤中的作用。方法采用Ⅶ胶原酶制作ICH模型,将90只雄性SD大鼠随机分为3组:正常对照组(10只)、假手术组(40只)和ICH模型组(40只)。分别在ICH后6 h、24 h、72 h和7 d四个时间点对大鼠进行神经功能缺损评分;用免疫组化及RT-q PCR来观察不同时间点脑组织NGAL及MMP-9表达情况。结果免疫组化及RT-q PCR结果显示ICH后模型组各时间点均可见大量NGAL、MMP-9阳性细胞及NGAL mRNA表达,且明显高于正常组及假手术组(P<0.05)。ICH模型组NGAL与MMP-9蛋白表达呈正相关(P<0.01)。ICH模型组NGAL、MMP-9蛋白表达均与大鼠神经功能缺损评分呈正相关(P<0.05)。结论 ICH后NGAL表达明显增加,提示NGAL可能参与了脑出血后继发性脑损伤,并可能通过调节MMP-9的活性从而发挥作用,但NGAL是否为MMP-9的上游调控因子尚有待进一步研究。
Objective To investigate the role of neutrophil gelatinase-associated lipocalin（ NGAL） in secondary brain injury after intracerebral hemorrhage（ ICH）. Methods Type VII collagenase was used to establish the model of ICH. A total of 90 male SpragueDawley rats were randomly divided into normal control group（ 10 rats）,sham-operation group（ 40 rats）,and ICH model group（ 40rats）. The neurologic impairment score was obtained at 6,24,and 72 hours and 7 days after ICH,and immunohistochemistry and RTq PCR were used to measure the expression of NGAL and matrix metallopeptidase-9（ MMP-9） in brain tissue at different time points.Results The results of immunohistochemistry and RT-q PCR showed that the model group had a large number of cells with the expression of NGAL and MMP-9,as well as the mRNA expression of NGAL,which were significantly higher than those of the normal control group and sham-operation group（ P〈 0. 05）. In the ICH model group,the protein expression of NGAL was positively correlated with that of MMP-9（ P 〈0. 01）,and the protein expression of NGAL and MMP-9 was also positively correlated with neurologic impairment score in rats（ P 〈0. 05）. Conclusions There is increased expression of NGAL after ICH,which suggests that NGAL may be involved in secondary brain injury after ICH. NGAL may regulate the activity of MMP-9,but further studies are needed to determine whether NGAL is the upstream regulatory factor of MMP-9.
作者
戴芳
王姗姗
许宏伟
DAI Fang WANG Shan-Shan XU Hong-Wei(Department of neurology, Xiangya Hospital of Central South University, Changsha 410008, China)
出处
《国际神经病学神经外科学杂志》
北大核心
2016年第4期292-296,共5页
Journal of International Neurology and Neurosurgery
