摘要
目的探讨靶向微泡携带尿激酶联合低频超声完全溶栓后远端组织微循环再栓塞对血栓近段动脉弹性功能的影响。方法 37只新西兰大白兔成功制作单侧股动脉血栓模型,靶向微泡携带尿激酶联合低频超声辅助照射下溶栓并股动脉血栓完全融通,应用僵硬度定量检测技术及应变技术测量单侧兔股动脉血栓近段大动脉的扩张系数、顺应系数、弹性系数、僵硬度、脉搏波传导速度(pulse wave velocity,PWV)及动脉内膜收缩期径向应变、径向应变率。取兔腓肠肌组织标本行组织病理检查,HE染色观察组织末端血管微小血栓形成情况,根据组织病理结果将兔分为微循环再栓塞组11只和微循环未栓塞组26只。结果微循环再栓塞组血栓近段动脉PWV[(8.087±0.487)m/s]高于微循环未栓塞组[(6.855±0.317)m/s)](P<0.05);微循环再栓塞组血栓近段动脉扩张系数[(0.037±0.008)/kPa]、顺应系数[(0.037±0.012)mm^2/kPa]、径向应变[(0.156±0.071)/s]、径向应变率[(0.040±0.024)%]较微循环未栓塞组[(0.057±0.005)/kPa、(0.057±0.031)mm^2/kPa、(0.244±0.059)/s、(0.059±0.032)%]减小,微循环再栓塞组弹性系数(5.155±0.563)、僵硬度(10.560±1.133)较微循环未栓塞组(3.857±0.366、7.906±0.737)增大,但差异均无统计学意义(P>0.05)。结论动脉血栓完全融通后引起的远端组织微循环再栓塞可引起血栓近段动脉以PWV为主的弹性功能减退,测量动脉PWV对远端组织微循环再栓塞有一定预示意义。
Objective To explore the influence of distal microcirculation re-embolism after targeted microbubhle carrying urokinase combined with low-frequency ultrasound thrombolysis on proximal arterial elastic function. Methods Thirty-seven New Zealand white rabbits were established unilateral femoral artery thrombosis models, and received targeted microbubble carrying urokinase combined with low-frequency ultrasound thrombolysis and complete femoral artery thrombosis. The distend coefficient, comply coefficient, elastic, stiffness, pulse wave velocity and radial strain and radial strain rate were measured by quantitive arterial stiffness and strain technique. The gastrocnemius muscle samples were obtained to observe the formation of distal vascular microthrombosis by HE staining. According to the pathological results, the rabbits were divided into thrombosis group （n= 11） and non-thrombosis group （n= 26）. Results The pulse wave velocity was significantly higher in thrombosis group （（8. 087 ± 0. 487） m/s） than that in non-thrombosis group （（6. 855±0. 317） m/s）） （P〈0.05）. The levels of distend coefficient （（0. 037± 0. 008） /kPa）, comply coefficient （（0. 037±0. 012） mm^2/kPa）, radial strain （（0. 156±0. 071）/s） and radial strain rate （（0. 040±0. 024） ±） were lower in thrombosis group than those in non-thrombosis （（0. 057±0. 005）/kPa, （0. 057±0. 031） mm2/kPa, （0. 244±0. 059） /s, （0. 059±0. 032）M）, and elastic and stiffness were higher in thrombosis group （5. 155 ± 0. 563, 10. 560 ± 1. 133） than those in non-thrombosis group （3. 857±0. 366, 7. 906±0. 737）, but there were no significant differences between two groups （P±0.05）. Conclusion Distal microcirculation re-embolism after thrombolysis could reduce the proximal arterial elastic function, and to measure pulse wave velocity has value to predicting distal microcirculation re-embolism.
出处
《中华实用诊断与治疗杂志》
2016年第11期1059-1061,共3页
Journal of Chinese Practical Diagnosis and Therapy
基金
国家自然科学基金(81301230)
关键词
微循环
再栓塞
动脉
僵硬度
靶向
脉搏波传导速度
兔
Microcirculation
re-embolism
artery
stiffness
targeted
pulse wave velocity
rats
