摘要
目的探讨老年溃疡性结肠炎(UC)患者外周血细胞因子信号传导抑制蛋白-3(SOCS-3)、肿瘤坏死因子-α(TNF-α)和一氧化氮(NO)水平与肠道菌群多样性的关系。方法选取29例老年UC患者作为研究对象,其中缓解期15例记为A组,活动期14例记为B组,另选取15名健康体检者作为对照组,分别测定三组受试者新鲜粪便中菌群结构和细菌数量及外周血SOCS-3、TNF-α及NO水平,观察老年UC患者外周血SOCS-3、TNF-α、NO水平变化与肠道菌群多样性的关系。结果三组受试者肠球菌和大肠杆菌数量比较差异无统计学意义(P>0.05),而B组双歧杆菌、乳酸杆菌数量以及SOCS-3水平均显著低于A组和对照组(P<0.05),TNF-α和NO水平均显著高于A组和对照组(P<0.05)。SOCS-3水平与乳酸杆菌呈正相关(r=0.347,P<0.05),与肠球菌呈负相关(r=-0.168,P<0.05);TNF-α和NO均与肠球菌呈正相关(r=0.426,P<0.05),与乳酸杆菌呈负相关(r=-0.356,P<0.05)。结论老年UC患者肠道菌群失衡会导致TNF-α和NO水平增加,SOCS-3水平降低,测定老年UC患者TNF-α、NO和SOCS-3水平可有效评估病情。
Objective To discuss the relationships between suppressor of cytokine signaling-3 （SOCS-3）, tumor necrosis factor-α（TNF-α） and nitric oxid （NO） levels in peripheral blood and intestinal flora diversity in elderly patients with ulcerative colitis （UC）. Methods Twenty-nine cases of elderly patients with UC treated in our hospital were selected, among which there were 15 cases in remission phase as the A group, and 14 cases in active phase as the B group;another 15 cases with the same age stage of healthy people for physical examination during the same period were selected as the control group. Three groups of subjects in fresh fecal flora and the number of bacteria and peripheral blood SOCS-3 , TNF-alpha and NO were measured. The relationships between the changes of levels of SOCS-3, TNF-αand NO in peripheral blood and the intestinal flora diversity were observed. Results There were no significant differences in the numbers of bacteria and Escherichia coli among the three groups （P〉0.05）. Bifidobacteria and lactobacilli in B group were significantly lower than those in the A group and the control group （P〈0.05）. The levels of TNF-αand NO in the B group were obviously higher than those in the A group and control group, while the level of SOCS-3 was significantly lower than those in the A group and control group （P〈0.05）. The level of SOCS-3 was positively correlated with the lactic acid bacterium （r=0.347, P〈0.05）, and was negatively correlated with the enterococcus （r=-0.168,P〈0.05）. The levels of TNF-α and NO were positively correlated with the enterococcus （r=0.426, P〈0.05）, and were negatively correlated with the lactic acid bacterium （r=-0.356, P〈0.05）. Conclusion The imbalance of intestinal flora in elderly patients with UC can lead to increased levels of TNF-αand NO, and decrease in SOCS-3 level. Therefore, the determination of TNF-α, NO and SOCS-3 levels in elderly patients with UC can effectively assess the severity of the disease.
出处
《热带医学杂志》
CAS
2016年第10期1283-1285,1326,共4页
Journal of Tropical Medicine
