摘要
以西芹鲜根为试材,采用丙酮浸提法,分离了西芹鲜根丙酮浸提液中的化感物质,并研究了化感物质对黄瓜枯萎病菌的抑制作用。结果表明:第5次层析抑制效果最佳的4个流分分别是RA32462、RA33444、RA98898、RA910664,其抑制率与丙酮对照相比分别提高了33.92%、35.80%、31.11%、32.15%。第6次层析抑制效果最佳的4个流分分别是RA324625、RA334448、RA988981、RA9106643,其抑制率与丙酮对照相比分别提高了35.10%、26.48%、34.06%、28.76%。通过对第6次层析的4个最佳流分的丙酮浸提物进行GC-MS检测,共鉴定出酸类、酯类、酚类和烷烃类共4类14种具有化感抑制作用的物质。
In this experiment,acetone extracts from fresh parsley roots were examined after the 5^th and 6^th cycles purification in column chromatography.The various column fractions were detected with F.oxysporum f.sp.cucumberinum.In terms of allelopathic inhibition effect and identified allelochemicals the experiment screened the best fractions of the 6^th chromatography using GC-MS.Four best fractions were selected by the 5^th extracts.They were RA32462,RA33444,RA98898,RA910664.The allelopathy inhibition effects compared with CK increased 33.92%,35.80%,31.11% and 32.15% respectively.And four best fractions were selected by the 6th extracts,they were RA324625,RA334448,RA988981,RA9106643.The allelopathic inhibition effects compared with CK increased 35.10%,26.48%,34.06%,28.76%respectively.There were 14 allelochemicals in four kinds which included acids,esters,phenols and alkanes were identified by GC-MS detection.
出处
《北方园艺》
CAS
北大核心
2016年第21期120-124,共5页
Northern Horticulture
基金
国家农业科技成果转化资金资助项目(2014GB2A400716)
内蒙古自治区应用技术研究与开发基金资助项目(20150136)
关键词
西芹鲜根
丙酮浸提物
黄瓜枯萎病菌
化感作用
化感物质
fresh parsley root
acetone extracts
Fusarium oxysporum f.sp.cucumerinum
allelopathy
allelochemicals
