摘要
目的:观察醇类物质及部分非离子表面活性剂对Trinder反应的影响。方法:以醇类物质及非离子表面活性剂溶液为样本,进行Trinder反应实验。肉眼观察颜色变化,用全自动生化分析仪记录光密度值。同时观察非离子表面活性剂对尿酸检测的影响。结果:以乙醇、乙二醇、异丙醇、Triton X-100、Triton X-405、Tween20和NP40为样本的Trinder实验均产生紫红色颜色反应。这些物质水溶液的光密度值均随浓度增加而增加,非离子表面活性剂的光密度水平与表面活性剂羟值有密切联系。含有0.1%Triton X-100、Triton X-405、Tween20和NP40的血清样本的尿酸水平明显高于三蒸水对照(P<0.01)。结论:醇类物质及部分非离子表面活性剂对Trinder反应有干扰。
Objective：To observe the effect of alcohol derivatives and some non-ionic surfactants on Trinder reaction.Methods：Using the aqueous solution of alcohol derivatives and non-ionic surfactant as sam-ple,Trinder reaction experiments were carried out.The color changes were observed with the naked eye,and the absorbance values were recorded using the automatic biochemical analyzer.Effects of nonionic surfactants on the detection of uric acid were also observed.Results：The results of Trinder reaction experiments using ethanol,ethylene glycol,isopropanol,Triton X-1 00,Triton X-405,Tween20 and NP40 as sample all pro-duced purple color reaction.The absorbance of these substances in aqueous solution increased with the in-crease of concentration,and the absorbance of ethylene glycol,TritonX-1 00,TritonX-405 and Tween20 changed more obviously with the concentration,the absorbance levels of non-ionic surfactants were closely re-lated to the hydroxyl values of the non-ionic surfactants.Levels of uric acid in serum samples containing 0.1%Triton X-1 00,Triton X-405,Tween20 and NP40 were significantly higher than that of samples contai-ning tri-distilled water.Conclusion：The alcohol derivatives and some non-ionic surfactants have the interfer-ence to the Trinder reaction.
出处
《江苏大学学报（医学版）》
CAS
2016年第5期433-436,共4页
Journal of Jiangsu University：Medicine Edition
