摘要
目的利用高效液相色谱建立肺结核丸的指纹图谱,为肺结核丸的质量控制和评价提供可靠的方法。方法采用HPLC梯度洗脱技术进行分离分析,采用Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.01%磷酸溶液,检测波长270 nm,体积流量0.8 m L·min-1,柱温25℃。结果 10个批次的肺结核丸有10个共有峰,鉴别了其中4个共有峰,初步确定了8个共有峰的归属,10批样品相似度均大于0.90。结论本实验所建立的方法简便快速,准确可靠,可以作为肺结核丸质量控制的有效方法。
OBJECTIVE To establish the HPLC fingerprints of Feijiehe pills and provide a foundation for its quality control and evaluation. METHODS A gradient elution method was applied. Ten batches of samples were analyzed by HPLC on a Diamonsil Cls , （4.6 mm x250 mm,5 txm） column with mobile phase composed of acetonitrile-0. 01% phosphoric acid solution at a flow rate of 0. 8 mL ·min-l, the detection was carried out at 270 rim, and the column temperature was maintained at 25 ℃. RESULTS There were 10 common peaks in the HPLC fingerprints. The similarity between peaks was more than 0. 90. CONCLUSION The method is sim- ple, accurate, reliable, and suitable to be applied to the quality control of Feijiehe pills.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2016年第21期1877-1879,共3页
Chinese Pharmaceutical Journal
