摘要
目的:比较溴化氰活化法和胺还原法制备A群和C群脑膜炎球菌多糖(group A and group C meningococcal polysaccharide,GAMP和GCMP)蛋白结合物的生化及免疫学特性。方法:以GAMP和GCMP为抗原,破伤风类毒素(tetanus toxoid,TT)为载体蛋白,采用溴化氰活化法和胺还原法制备结合物,并对其进行生化及免疫学检测。结果:两种方法均可将多糖与TT有效结合,在小鼠体内均具有良好的免疫原性。溴化氰活化法制备的结合物收率、高分子结合物含量、免疫小鼠产生的抗体效价更高。结论:采用溴化氰活化法制备GAMP-TT,GCMP-TT结合物优于胺还原法。
Objective： To compare the biochemical and immunological characteristics of tetanus toxoid（ TT） conjugated group A and group C meningococcal polysaccharide（ GAMP and GCMP） prepared by cyanogen bromide activation and amine reduction, respectively. Methods： GAMP-TT and GCMP-TT conjugates were prepared by cyanogens activation and amine reduction using GAMP and GCMP as polysaccharide antigens and TT as carrier protein. Their biochemical and immunological characteristics were analyzed. Results： Polysaccharides and TT were bound effectively by the two methods,and the resulted conjugates showed good immunogenicity in NIH mice. The GAMP-TT and GCMP-TT prepared by cyanogen bromide activation had higher yield,and higher molecular conjugate content and the antibody titer than those prepared by amine reduction. Conclusion： Cyanogen bromide activation is more suitable for the preparation of GAMP-TT and GCMP-TT than amine reduction.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2016年第21期2441-2445,共5页
Chinese Journal of New Drugs
基金
国家"重大新药创制"科技重大专项资助项目(2013ZX09402302)
关键词
脑膜炎球菌多糖
结合疫苗
结合方法
meningococcal polysaccharide
conjugate vaccine
conjugation methods
