摘要
采用固相萃取结合高效液相色谱技术检测食品中的吡咯素。食品原料采用OasisTMHLB固相萃取柱进行预富集,并用HPLC法进行检测。结果表明,吡咯素在5×10^(-7)～2×10^(-3)mol/L浓度范围内呈现良好的线性关系,相关系数R^2=0.9999,RSD为0.78%～7.51%,最低检出限为5×10^(-7)mol/L(S/N=3)。本方法操作简单,灵敏度高,可应用于食品中痕量吡咯素的检测。
A solid phase extraction-high performance liquid chromatography for determination of pyrraline in food was established. Pyrraline was extracted from food material by a HLB-SPE column and analyzed by high performance liquid chromatography.Results showed that a good linear range from 5 ×10^-7 to 2×10^-3 mol/L with correlation coefficient of 0.9999 was obtained. The relative standard deviation were 0.78% ~7.51%.The limit of detection was 5 ×10^-7 mol/L（S/N = 3）.The discussed method was simple and accurate and could be applied to the detection of trace pyrraline in food.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第22期90-93,102,共5页
Science and Technology of Food Industry
基金
国家自然科学基金(31501559,31571940)
关键词
高效液相色谱
固相萃取
吡咯素
检测
high performance liquid chromatography
solid phase extraction
pyrraline
detection
