摘要
目的探讨中药白芷活性成分欧前胡素增强顺铂对口腔鳞癌干细胞的杀伤活性及其机制。方法流式细胞术检测欧前胡素和顺铂对Cal27细胞系的肿瘤干细胞种群比例的影响。MTT法和Annexin V染色法检测欧前胡素对顺铂杀伤Cal27肿瘤干细胞能力的影响。利用Western blot方法验证欧前胡素是否调节Cal27肿瘤干细胞MCL-1的表达。运用JC-1染色、Annexin V染色及Western blot方法研究欧前胡素影响顺铂疗效的信号通路。结果顺铂组Cal27细胞系的肿瘤干细胞种群比例显著高于对照组(19.4%比8.5%,P<0.05),而顺铂联合欧前胡素组后肿瘤干细胞种群比例下降至11.5%(P<0.05)。顺铂联合欧前胡素组对Cal27肿瘤干细胞的细胞活力的抑制率为(57.4±3.4)%,凋亡诱导率为(36.7±2.1)%,均显著高于欧前胡素组的(7.9±6.6)%与(4.2±0.4)%(P<0.05)和顺铂组的(11.8±0.8)%与(8.4±0.5)%(P<0.05)。顺铂联合欧前胡素组Cal27肿瘤干细胞的线粒体膜电位显著低于欧前胡素组和顺铂组(0.37±0.02比0.96±0.04、0.86±0.03,P<0.05)。顺铂联合欧前胡素组处理的Cal27肿瘤干细胞的caspase-9和caspase-3的活化水平分别为0.48±0.03、0.56±0.04,显著高于欧前胡素组的0.03±0.01(P<0.05)、0.03±0.01(P<0.05)和顺铂组的0.10±0.02(P<0.05)、0.12±0.02(P<0.05)。与对照组比较,欧前胡素处理后Cal27肿瘤干细胞的MCL-1表达水平显著降低(0.29±0.02比0.89±0.05,P<0.05),而顺铂对MCL-1的表达无影响(0.90±0.05比0.89±0.05,P>0.05)。结论欧前胡素可通过下调MCL-1的表达提高口腔鳞癌干细胞对顺铂的敏感性。
Objective To investigate the role of imperatorin in cisplatin-induced cell death in oral cancer stem cells and the underlying mechanism. Methods The percentage of cancer stem cell population in the Cal27 cell line treated with imperatorin and cisplatin was assessed by flow cytometry. MTT assay and Annexin V staining was performed to evaluate the effect of imperatorin on the cisplatin-induced cell death and apoptosis in the Cal27 cancer stem cells. Results The Cal27 cancer stem cell population in single cisplatin treatment group was significantly higher than that in control group（19.4% vs 8.5%, P〈0.05）, but lower than 11.5% of cisplatin plus imperatorin group（P〈0.05）. The inhibitory rate of cell viability and the apoptotic rate of Cal27 cancer stem cells in cisplatin plus imperatorin group was 57.4%±3.4% and 36.7%±2.1%, respectively, which were significantly higher than those in single cisplatin group（ 7.9%±6.6% and 4.2%±0.4%, P〈0.05） and single imperatorin group（11.8%±0.8% and8.4% ±0.5%, P〈0.05）. The mitochondrial transmembrane potential of Cal27 cancer stem cells in cisplatin plus im peratorin group was significantly lower than that in single cisplatin group and single imperatorin group（0.37±0.02 vs 0.96±0.04, 0.86±0.03, all P〈0.05）. The relative expression of cleaved caspase-9 and caspase-3 in cisplatin plus imperatorin group was significantly higher than that in single cisplatin group and imperatorin group（caspase-9：0.48±0.03 vs 0.03±0.01, 0.10±0.02; caspase-3： 0.56±0.04 vs 0.03±0.01, 0.12±0.02; all P〈0.05）. Treatment with im peratorin significantly decreased the expression of MCL-1（0.29±0.02 compared with 0.89±0.05 in control group, P〉0.05）,whereas the cisplatin treatment did not change the MCL-1 level（0.90 ±0.05 vs 0.89±0.05, P〈0.05）. Transfection with MCL-1 vector significantly impaired the synergistic effect of imperatorin on the cisplatin-induced cell death and apoptosis in the Cal27 cancer stem cells. Conclusion Imperatorin increased the sensitivity of oral cancer stem cells to cisplatin via down-regulating the expression of MCL-1.
出处
《浙江中西医结合杂志》
2016年第11期991-995,共5页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
