摘要
这研究被设计与荧光决定帮助计算机的精子 morphometry 分析(CASA-Morph ) 的能力在在牛的种类从产生的原子 morphometrics 和不同统计过程带不同的性染色体的精子之间区别。学习被划分成二个实验。第一是学习 X-chromosome-bearing 和 Y-chromosome-bearing 精子之间的 morphometric 差别(SX 和 SY，分别地) 。从八头公牛的精子被处理同时由基于荧光的 CASA-Morph 由鱼和精子 morphometry 估计性染色体。SX 房间平均比 SY 房间大(P <；0.001 ) 尽管与公牛之间的重要差别。由判别式分析的所有测量特征的同时的评估表明那个原子区域和平均荧光紧张是逐步的判别式功能分析作为在 SX 和 SY 之间的最好的辨别者选择的变量。在第二个实验，精子从在 nonsexed (混合 SX 和 SY ) 和精液从四头公牛取样的 sexed (SX ) 的 CASA-Morph 的原子 morphometric 结果被比较。鱼根据他们的性染色体内容允许精子的一个成功的分类。X 性精子比 nonsexed 精子显示了一种更大的尺寸和荧光紧张(P <；0.05 ) 。我们断定 CASA-Morph 基于荧光的方法有潜力尽管更多的研究被需要由这种技术增加性决心的精确，在牛的种类发现 X-chromosome-bearing 和 Y-chromosome-bearing 精子之间的差别。
This study was designed to determine the ability of computer-assisted sperm morphometry analysis （CASA-Morph） with fluorescence to discriminate between spermatozoa carrying different sex chromosomes from the nuclear morphometrics generated and different statistical procedures in the bovine species. The study was divided into two experiments. The first was to study the morphometric differences between X- and Y-chromosome-bearing spermatozoa （SX and SY, respectively）. Spermatozoa from eight bulls were processed to assess simultaneously the sex chromosome by FISH and sperm morphometry by fluorescence-based CASA-Morph. SX cells were larger than SY cells on average （P 〈 0.001） although with important differences between bulls. A simultaneous evaluation of all the measured features by discriminant analysis revealed that nuclear area and average fluorescence intensity were the variables selected by stepwise discriminant function analysis as the best discriminators between SX and SY. In the second experiment, the sperm nuclear morphometric results from CASA-Morph in nonsexed （mixed SX and SY） and sexed （SX） semen samples from four bulls were compared. FISH allowed a successful classification of spermatozoa according to their sex chromosome content. X-sexed spermatozoa displayed a larger size and fluorescence intensity than nonsexed spermatozoa （P 〈 0.05）. We conclude that the CASA-Morph fluorescence-based method has the potential to find differences between X- and Y-chromosome-bearing spermatozoa in bovine species although more studies are needed to increase the precision of sex determination by this technique.
