摘要
目的 建立同时测定复方三七胶囊中人参皂苷Rg1、Re、Rb1和三七皂苷R1的UPLC方法。方法 采用UPLC,ACQUITY BEH C18色谱柱（2.1mm×100mm,1.7μm）,乙腈-水为流动相梯度洗脱,流速为0.45m L/min,检测波长为203nm,柱温为25℃。结果 人参皂苷Rg1、Re、Rb1和三七皂苷R1分别在19.61~392.2μg/m L（r=0.9997）、4.813~96.26μg/m L（r=0.9998）、19.92~398.4μg/m L（r=0.9998）和4.850~97.00μg/m L（r=0.9999）线性关系良好,平均加样回收率（n=9）分别为100.00%、99.78%、99.92%和99.83%,RSD分别为0.30%、0.45%、0.29%和0.54%。结论 方法快速有效,适用于测定复方三七胶囊中人参皂苷Rg1、Re、Rb1和三七皂苷R1含量。
Objective To determine ginsenoside Rg1, Re, Rb1 and notoginsenoside R1 in Fufang Sanqi capsule by UPLC. Methods UPLC was performed on a ACQUITY BEH C18 column（2.1mm＆#215;100mm, 1.7μm） at 25℃ . Acetonitrile- water gradient elution was applied at the flow rate of 0.45ml /min. The detection wavelength was 203nm. Results Ginsenoside Rg1, Re, Rb1 and notoginsenoside R1 were thoroughly separated with the related substances. The linear ranges were 19.61 ~ 392.2μg/mL（r=0.9997）, 4.813 ~ 96.26μg/mL（r=0.9998）,19.92 ~ 398.4 μg/mL （r=0.9998） and 4.850 ~ 97.00μg/mL（r=0.9999）, the average recoveries （n=9） were 100.00%, 99.78%, 99.92% and 99.83% with RSD of 0.30%, 0.45%, 0.29% and 0.54%. Conclusion The method is fast, efficient, and it is suitable for determining ginsenoside Rg1, Re, Rb1 and notoginsenoside R1 in Fufang Sanqi capsule.
出处
《中国医药科学》
2016年第17期33-36,共4页
China Medicine And Pharmacy
