摘要
目的研究中药治疗对少弱畸形精子症患者的精子染色体非整倍体率及辅助生殖技术(assisted reproductive technology,ART)结局的影响。方法将58例要求接受ART的少弱畸形精子症患者随机分为试验组和对照组。试验组26例,采用生精汤治疗3个月后进行精子染色体分析,再接受辅助生殖治疗;对照组32例,直接进行辅助生殖治疗,2组均采用卵胞质内单精子显微注射(intracytoplasmic sperm injection,ICSI)受精方式,观察2组的受精率、卵裂率、优胚率、可用胚胎率、妊娠率及流产率。结果治疗前,试验组和对照组的精子非整倍体率分别为2.3%和2.1%,差异无统计学意义。治疗后,试验组经复查,精子染色体非整倍体率为1.7%,与治疗前相比,差异有统计学意义(P<0.05);试验组和对照组的受精率分别为82.55%和80.80%,卵裂率分别为99.25%和98.77%,优胚率分别为24.91%和24.07%,可用胚胎率分别为76.23%和70.68%,妊娠率分别为57.69%和31.25%,流产率分别为7.70%和12.50%,种植率分别为38.98%和22.20%,其中妊娠率和种植率2组间差异有统计学意义(P<0.05)。结论中药治疗可降低精子染色体非整倍体率,改善辅助生殖技术的结局。
Objective To study the influence of Chinese medicine on the rate of sperm aneuploidy and assisted reproductive technology（ART） result. Methods 58 infertile men with oligoasthenoterazoospermia who received ART treatment were divided into experimental group（26 cases）and control group（32 cases）. The experimental group received ART treatment after 3 course of Chinese medicine. The control group received ART treatment di- rectly. Fluorescence in situ hybridization（FISH）was used to analyze spermatozoa chromosomes. The rate of fertili- zation,the rate of cleavage,the rate of high embryo,he rate of useful embryo,the rate of pregnancy,the rate of a- bortion and the rate of implantation were observed and compared between two groups. Results Before treatment, the rate of sperm aneuploidy for experimental group and control group was 2.3% and 2. 1% respectively,with no significant difference. After treatment,the rate of sperm aneuploidy for experimental group was 1.7 % which was significant lower than before （P〈0.05）. For experimental group and control group, the rate of fertilization was 82. 55%,80.80%,the rate of cleavage was 99.25%,98. 77%,the rate of high embryo was 24. 91%,24.07%,the rate of useful embryo was 76. 23% ,70. 68% ,the rate of pregnancy was 57. 69%,31.25% ,the rate of abortion was 7. 70% ,12.50% ,the rate of implantation was 38.98% ,22.20%. There was significant difference for the rate of pregnancy and implantation between two groups（both P〈0.05）. Conclusion Chinese medicine can reduce the rate of sperm aneuploidy and improve ART result.
作者
韩临晓
孙晓岩
陈博
郑志涛
钟秋生
潘瑞华
HAN Linxiao SUN Xiaoyan CHEN Bo et al(Center of Reproductive Medicine, the Third People＇s Hospital of Dongguan , Dongguan 523326, Chin)
出处
《中西医结合研究》
2016年第5期225-228,共4页
Research of Integrated Traditional Chinese and Western Medicine
基金
东莞市科技计划医疗卫生类科研一般项目(No.东科[2014]104号之232)
