摘要
为研究罗非鱼竖鳞病病原菌的致病性,本试验从出现竖鳞症状的患病罗非鱼不同部位分离得到1株病原菌,命名为GXKJDX01,采用16SrDNA基因序列分析、细菌的形态学观察、生理生化鉴定、药敏试验和人工感染试验的方法对其研究。结果显示,致病菌株GXKJDX01为维氏气单胞菌(Aeromonas veronii);该菌株对青霉素G、红霉素、万古霉素等5种药物有高度的耐药性;对罗非鱼、黄颡鱼、泥鳅和禾花鲤均有较强的致病性。使用恩诺沙星、左氧沙星、头孢曲松等11种药物可有效对其进行防制。本试验首次报道维氏气单胞菌作为竖鳞病的案例,为其有效防控提供理论依据。
In order to study the pathogenicity of the pathogenic bacteria in the vertical scale disease of Tilapia,one strain of pathogenic bacteria was isolated from different parts of the diseased Tilapia,named as GXKJDX01.Then the methods of 16 SrDNA gene sequence analysis,bacterial morphology observation,physiological and biochemical identification,drug sensitivity test and artificial infection test were used.The results showed that the pathogenic strain GXKJDX01 was Aeromonas veronii,strain GXKJDX01 was not sensitive to 5 medicines,including penicillin G,erythromycin,vancomycin,and had a strong pathogenic effect in yellow-head catfish meat,Tilapia,loach and Procypris merus.11 kinds of drugs,including of enrofloxacin,levofloxacin,ceftriaxone,could be effective to the prevention and control.This study is the first report on the case of the pathogenic bacteria of the lepidorthosis,which was the Aeromonasveronii,and the theoretical basis was provided for the effective prevention and control.
作者
韦阳道
杨军
文衍红
司徒玲
刘霞
易弋
WEI Yang-dao YANG Jun WEN Yan-hong SI Tu-ling LIU Xia YI Yi(College of Biological and Chemical Engineering, Guangxi University of Science and Technology , Liuzhou 545006, China Guangxi Key Laboratory of Green Processing of Sugar Resources, C-uangxi University of Science and Technology , Liuzhou 545006, China Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology , Liuzhou 545006, China Liuzhou Fishery Technical Extension Station, Liuzhou 545006, China)
出处
《中国畜牧兽医》
CAS
北大核心
2016年第11期3047-3052,共6页
China Animal Husbandry & Veterinary Medicine
基金
现代农业产业技术体系专项资金项目(CARS-49)
广西水产畜牧业科技项目(桂渔牧财[2015]28号)
