摘要
目的:研究miRNA-203在甲状腺乳头状癌组织中的表达水平及其与癌组织临床病理参数的相关性,并探讨其在甲状腺乳头状癌WRO细胞增殖过程中的作用。方法:收集2013-2016年30例甲状腺乳头状癌组织及其配对癌旁组织病理标本,采用荧光定量PCR方法检测甲状腺癌组织和癌旁组织中miRNA-203的表达水平,并分析其与临床病理参数的相关性;同时转染miRNA-203mimics调控WRO细胞株,用MTT、流式分析和Western blot方法分析WRO细胞的增殖能力、周期改变及相关周期蛋白(CyD1、CyB1)的变化。结果:相对于癌旁组织,甲状腺乳头状癌组织中miRNA-203显著低表达;在WRO细胞中转染miRNA-203mimics后,可显著抑制细胞增殖能力,阻滞G2/M期,同时上调CyB1、下调CyD1周期相关蛋白的表达。结论:甲状腺乳头状癌组织中miRNA-203可能起着抑癌作用,与甲状腺乳头状癌细胞的增殖能力相关,可能是甲状腺乳头状癌干预的新靶点之一。
Objective：To investigate the expression of miRNA-203 in papillary thyroid carcinoma（PTC）tissues and its correlation with clinical pathological parameters,explore its effect on cell proliferation of WRO cell.Method：Thirty cases of PTC tissues,paired normal tissues were collected in our hospital during 2013-2016.The expression of miRNA-203 was determined by qRT-PCR,then the relationship of miRNA-203 expression,clinical pathological parameters were analyzed.WRO cells were transfected with miRNA-203 mimics,then cell proliferation,cell cycle and concerned cyclin protein（CyD1,CyB1）were tested by MTT,flow cytometry and western blot.Result：Compared to the paired normal tissues,tumor tissues showed sifnificantly lower expression of miRNA-203.Upregulaion of miRNA-203 in WRO cells effectively reduced cell growth,G2/M arrest.Mechanistically,in the miRNA-203-mimics-treated groups,cell-cycle-related proteins cyclin B1 was up-regulated,while cyclin D1 was down-regulated.Conclusion：miRNA-203 may play an anticarcinogenic effect in PTC.Upregulation of miRNA-203 is highly correlated with cell prolliferation,and maybe miRNA-203 is a potential targert for the treatment of thyroid carcinoma.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
北大核心
2016年第22期1769-1773,共5页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
