摘要
目的:建立适合DNA条形码分析的镰形棘豆DNA提取方法。方法:以镰形棘豆(Oxytropis falcate Bunge)叶片为材料,采用改良的DNA提取方法对其DNA进行提取,并用琼脂糖凝胶电泳和PCR扩增对提取的DNA进行检测。结果:本方法提取的DNA,能够满足以PCR为基础的分子鉴定的基本要求。操作简便,需时短,成本低廉,适于DNA的大批量提取。结论:本方法适合DNA条形码研究的DNA提取。
Objective：To establish a DNA extraction method from LianXing JiDou（Oxytropis falcate Bunge）for DNA bar code analysis.Methods：Leaves of LianXing JiDou were used to extract DNA by using a modified DNA method;agarose gel electrophoresis and PCR amplification were used to detect extracted DNA.Results：The DNA extracted by this method could meet basic requirements for PCR molecular identification.The method is simple,time-saving,and low-cost;it is suitable for extraction in large quantities.Conclusion：This method is a proper method of DNA extraction for bar code analysis.
出处
《西部中医药》
2016年第9期34-36,共3页
Western Journal of Traditional Chinese Medicine
基金
甘肃省中医药科研立项课题(编号GZK-2012-71)
甘肃省中医院博士启动基金项目(编号博2013-9)
