摘要
目的:比较栀子柏皮汤3种方法(半仿生提取法、醇提取法、水提取法)提取液的成分,为栀子柏皮汤提取提供实验依据。方法:在饮片规格、溶剂用量、以及提取、滤过、浓缩设备相同的条件下,将栀子柏皮汤分别以半仿生提取法、醇提法、水提法3种方法提取,以HPLC测定3种方法提取液中主要有效成分栀子苷、盐酸小檗碱、甘草次酸含量;以紫外分光光度法测定总生物碱含量;将栀子苷、盐酸小檗碱、甘草次酸、总生物碱含量以及相对分子质量≤1 000 Da提取物得率共5个指标测定结果经标准化处理后进行加权求和,得综合评判值Y并进行比较,Y大者为优。结果:栀子柏皮汤3种方法提取液的综合评价值Y大小顺序依次为:半仿生提取液>醇提液>水提液。结论:栀子柏皮汤3种提取方法中,以半仿生提取法最佳。
Objective： To compare the contents of the active compositions from Zhizi Bopi Tang extracted by three methods of semi-bionic extraction method（SBE method）,alcohol extraction method（AE method）,water extraction method（WE method） and provide experimental evidence for extracting Zhizi Bopi Tang. Method： Zhizi Bopi Tang was extracted by three methods of SBE method,AE method and WE method under the same conditions in the size of decoction pieces,amount of solvent,and the same extraction,filtration and concentration equipment. The contents of gardenoside,berberine and glycyrrhetinic acid in the extracts under the above 3 methods were determined by HPLC method and the content of total alkaloids was determined by ultraviolet spectropho-tometric method. The comprehensive evaluation value Y was based on the weighted sum of processed data of gardenoside content,berberine content,glycyrrhetinic acid content,total alkaloids content and the extracts with molecular weight ≤ 1 000 Da,the samples with larger Y value were better. Result： The order of the comprehensive evaluation Y of the three methods was as follows： SBE extracting solution〉 AE extracting solution 〉WE extracting solution. Conclusion： It can be concluded that SBE method was the best one among the three methods for Zhizi Bopi Tang.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第22期59-62,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技部国际合作项目(2007DFA31180)
关键词
栀子柏皮汤
半仿生提取
多指标
综合评判
分子量≤1
000
Da提取物
Zhizi Bopi Tang
semi-bionic extraction method
multi-component quantitation
comprehensive evaluation
molecular weight≤1 000 Da
