摘要
目的:观察五味子多糖对5-氟尿嘧啶(5-FU)引起的肠道黏膜炎的治疗作用及探讨其作用机制。方法:使用5-FU造模,将小鼠随机分为正常组,模型组,多糖高、中、低剂量组(20,10,5 mg·kg-1)。观测小鼠腹泻和体重情况;将小鼠处死后,通过测量小肠绒毛高度、隐窝深度、以及计算绒毛高度/隐窝深度比值进行形态学评价;通过酶联免疫(ELISA)法测定小肠组织白细胞介素-1β(IL-1β),IL-6,肿瘤坏死因子-α(TNF-α)的含量。结果:给药第4天,与模型组相比,高、中剂量多糖组体重比率明显提高(P<0.01);高、中剂量多糖组大便得分显著低于模型组(P<0.05,P<0.01)。五味子多糖显著抑制5-FU引起的小肠肠绒毛高度缩短、隐窝深度值更大、绒毛高度/隐窝深度比减少(P<0.01);明显降低TNF-α,IL-1β,IL6水平(P<0.01)。结论:五味子多糖能改善5-FU引起的肠道黏膜炎症状,其作用机制可能与通过抑制TNF-α,IL-1β,IL6等炎症因子的水平有关。
Objective： To observe the therapeutic effect of Schisandrae Chinensis Fructus polysaccharide（SCFP） on 5-fluorouracil（5-FU） induced gastrointestinal mucositis and explore the underlying mechanism. Method： 5-FU was used to establish models and the mice were randomly divided into normal group,control group,low-dose polysaccharide（LDP） group,middle-dose polysaccharide（MDP） group and high-dose polysaccharide（HDP） group（5,10,and 20 mg·kg- 1respectively）. Body weight and scoring for diarrhoea index were recorded. After the mice were sacrificed,small intestinal villous height and crypt depth were measured for orphological evaluation; the levels of tumor necrosis factor-α（TNF-α）,interleukin-6（IL-6）,interleukin-1β（IL-1β） in the intestinal tissues were determined by ELISA. Result： On day 4 of treatment,as compared with the control group,the body weight loss in MDP group and HDP group was inhabited significantly（P 〈0. 01）;diarrhoea scores of mice in MDP group and HDP group were significantly decreased（P 〈0. 05,P 〈0. 01）. SCFP significantly inhibited the shortening of villus heights,enlarging of crypts depths and redecreasing of villus heights /crypts depths ratio in small intestine induced by 5-Fu（P〈 0. 01）. The levels of TNF-α,IL-1β,and IL-6 in mice treated with SCFP were significantly decreased in comparison with the normal group（P 〈0. 01）. Conclusion： Our results suggest that SCFP could improve the symptoms of gastrointestinal mucositis induced by 5-FU,and the mechanism may beassociated with inhibiting the levels of TNF-α,IL-6 and IL-1β.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第22期124-128,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81302958
81373707)
广州市产学研重大专项(2014Y2-00504)
关键词
五味子多糖
5-氟尿嘧啶
肠道黏膜炎
Schisandrae Chinensis Fructus polysaccharide
5-fluorouracil
gastrointestinal mucositis
