摘要
目的建立反相高效液相色谱法测定桃金娘果酒中原儿茶酸、儿茶素、咖啡酸、阿魏酸和白藜芦醇5种酚类物质的含量。方法以乙酸乙酯为萃取溶剂对桃金娘果酒中的酚类物质进行萃取,采用Phenemenex C18（4.6 mm×250 mm,5μm）反相色谱柱分离,以0.4%甲酸-甲醇为流动相进行梯度洗脱,流速为1.0 mL/min,柱温为30℃。采用紫外检测器检测,检测波长为280 nrm。结果原儿茶酸、儿茶素、咖啡酸、阿魏酸、白藜芦醇在0.012-0.20 mg/mL范围内均呈良好线性关系（r≥0.9993）,加标回收率在98.9%-100.9%范围,相对标准偏差小于2.10%。结论该方法操作简单,灵敏度高,可适用于桃金娘果酒中5种酚类物质的含量测定。
Objective To establish a method for the determination of 5 kinds of polyphenols including protocatechuic acid,resveratrol,caffeic acid,ferulic acid and catechins in fruit wine of myrtle by reversed phase high performance liquid chromatography（RP-HPLC）.Methods Polyphenols were extracted with ethyl acetate,separated by the analytical column of Phenemenex C18（4.6 mm×250 mm,5 urn） with the mobile phase of methanol and 0.04%methanol acid water for gradient elution at the flow rate of 1.0 mL/min,and the column temperature was30 ℃,the wavelength of UV detector was 280 nm.Results Protocatechuic acid,resveratrol,caffeic acid,ferulic acid and catechins showed good linear relationships（r≥0.9993） within the ranges of 0.012-0.20 mg/mL,the recoveries ranged from 98.9%to 100.9%with RSDs〈2.1%.Conclusion The method is sensitive and accurate,which can be applied for determination of polyphenols in myrtle fruit wine.
出处
《食品安全质量检测学报》
CAS
2016年第9期3800-3804,共5页
Journal of Food Safety and Quality
关键词
果酒
酚类物质
原儿茶酸
儿茶素
咖啡酸
阿魏酸
白藜芦醇
反相高效液相色谱法
fruit wine
polyphenols
protocatechuic acid
resveratrol
caffeic acid
ferulic acid
catechins
reversed phase high performance liquid chromatography
