摘要
目的:建立安神补脑胶囊中淫羊藿苷含量的测定方法。方法:采用高效液相色谱法,C_(18)柱(4.6 mm×250 mm,5μm);流动相:乙腈-0.1%三氟乙酸(30∶70);检测波长:270 nm;流速:1.0 m L/min。结果:淫羊藿苷在0.050 6~2.024 0μg范围内有良好的线性关系(r^2=0.999),平均回收率为96.4%,RSD=0.56%。结论:该方法准确、快速、简便,可用于安神补脑胶囊中淫羊藿苷的含量测定。
Objective：The method for determining icariin in Anshen Bunao capsule was established. Methods：HPLC was conducted on Packing C1s column （4.6 mm×250 mm,5 μm）with the mobile phase of acetonitrile and 0.1% trifluoroacetic acid （30：70）. Detection wavelength was at 270 nm and flow rate was 1.0 mL/min. Results：The linear range of icariin was 0.050 6-2.024 0 μg （r2=0.999）, and the average recovery was 96.4% （RSD=0.56%）. Conclusion：The method is simple, rapid, accurate and can be used in the determination of icariin in Anshen Bunao capsule.
出处
《山西中医学院学报》
2016年第5期29-30,38,共3页
Journal of Shanxi College of Traditional Chinese Medicine
