摘要
[目的]通过对黄淮地区大豆种子带菌的快速检测,了解该地区大豆种子携带病原菌的情况。[方法]采用环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)检测黄淮地区29个大豆主栽品种种子上平头炭疽菌、胶孢炭疽菌、尖镰孢菌、木贼镰孢菌、层出镰孢菌、黄色镰孢菌、禾谷镰孢菌、茄腐镰孢菌、大豆拟茎点种腐病菌、立枯丝核菌、大豆炭腐病菌、冬青丽赤壳菌、大豆疫霉病菌等大豆主要病原菌的携带情况。[结果]供试的29个品种中有20个品种的种子样本检出携带上述病原菌,分别携带所检测的13种病原菌中的7种,检出率由高到低依次为平头炭疽菌、大豆拟茎点种腐病菌、木贼镰孢菌、黄色镰孢菌、尖镰孢菌、胶孢炭疽菌和层出镰孢菌,但不同大豆品种种子携带病原菌的种类及数量有较大差异,其中品种‘漯39001’检出的病原菌多达6种。[结论]本研究为大豆种子带菌检测提供了新的方法,并揭示了黄淮地区大豆种子携带病原菌的状况,在生产上有较大的应用价值。
[ Objectives ] In order to know the situation of soybean seed-borne pothogens in Huang-huai area, the soybean seed-borne pathogens were detected using loop-mediated isothermal amplification （LAMP）assays. [ Methods ] In this research, 13 species of seed-borne pothogens on soybean seed samples of 29 cultivars in Huang-huai area were detected using LAMP method, including Colletotrichum truncatum , C. gloeosporioide , Fusarium oxysporum , F. equiseti , F. proliferatum , F. culmorum , F. graminearurn , F. solani , Phomapsis longicolla , Rhizoctonia solani , Macrophomina phaseolina , Calonectria ilicicola , and Phytophthora sojae. [ Results ] There are 20 soybean cultivars earring 7 of these seed-borne pothogens, and the dominant population is C. truncatum, followed by P. longicolla, F. equiseti , F. culmorum , F. oxysporum , C. gloeosporioide , and F. proliferatum. The pathogens carried with seeds of different cuhivars are various, and the number of pathogens detected changes from 1 to 6. [ Conclusions ] A simple, rapid and sensitive method for detecting soybean seed-borne pothogens was developed in this study, and the situation of soybean seed-borne pothogens in Huang- huai area was revealed with the method.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2016年第6期947-953,共7页
Journal of Nanjing Agricultural University
基金
国家公益性行业(农业)科研专项(201303018)
国家863计划项目(2012AA101501)
关键词
大豆
种子带菌
环介导等温扩增技术
快速检测
soybean
seed-borne pothogens
loop-mediated isothermal amplification （LAMP）
rapid detection
