摘要
目的：探讨同源异型盒基因 B1（HOXB1）与微小 RNA-3175（miR-3175）在人胶质瘤中表达的关系及临床意义。方法通过实时荧光定量 PCR（qRT-PCR）检测60例人胶质瘤组织和15例正常脑组织中 HOXB1与 miR-3175的表达，采用 Spearman 相关分析法分析人胶质瘤组织中 HOXB1、miR-3175表达的相关性，分析 HOXB1、miR-3175表达与临床病理特征的关系，采用 Kaplan-Meier 评估HOXB1、miR-3175与患者生存期的关系，采用 COX 回归模型分析患者预后因素。结果较正常脑组织， HOXB1在人胶质瘤组织中低表达（1．498±0．323∶0．946±0．588，t ＝－5．680，P ＝0．000），miR-3175在人胶质瘤组织中高表达（1．008±0．355∶2．076±0．841，t ＝4．274，P ＝0．000），并且在人胶质瘤组织中HOXB1与 miR-3175表达呈负相关（r ＝－0．601，P ＝0．000）。HOXB1表达与肿瘤分级相关（χ2＝4．848， P ＝0．028），miR-3175表达与肿瘤分级（χ2＝5．640，P ＝0．018）、Karnofsky 功能状态评分（χ2＝4．785，P ＝0．029）相关。Kaplan-Meier 分析结果显示 HOXB1、miR-3175高表达组与低表达组中位生存期[HOXB1：（21．0±4．0）个月∶（7．0±0．8）个月；miR-3175：（6．0±0．6）个月∶（16．0±5．8）个月]差异有统计学意义（χ2＝7．495，P ＝0．006；χ2＝9．591，P ＝0．002）。COX 回归模型分析结果显示肿瘤分级（RR ＝6．556，95％CI 为1．196～35．952，P ＝0．002）、HOXB1（RR ＝0．018，95％CI 为0．001～0．312，P ＝0．006）和miR-3175（RR ＝2．098，95％CI 为1．663～7．513，P ＝0．037）是影响胶质瘤患者预后的独立因素。结论HOXB1与 miR-3175在人胶质瘤中的表达呈负相关，并与胶质瘤组织的恶性程度、胶质瘤患者的生存期及预后密切相关。
Objective To explore the relationship and the clinical significance of homeobox gene B1 （HOXB1 ）and microRNA-31 75 （miR-31 75）expressions in human glioma.Methods The expression levels of HOXB1 and miR-31 75 in 60 glioma tissues and 1 5 normal brain tissues were analyzed by real-time fluores-cent quantitative PCR （qRT-PCR）.Spearman rank correlation analysis was performed to explore the relation-ship between HOXB1 and miR-31 75 in human glioma tissues.The relationship between HOXB1 （or miR-31 75）and clinical pathological characteristics of glioma patients was analyzed.The correlation of HOXB1 （or miR-31 75）and survival rate was calculated by Kaplan-Meier.And COX regression models were used to assess the prognostic factors.Results Compared with normal brain tissues,the expression of HOXB1 was significant-ly decreased in glioma tissues （1 .498 ±0.323 vs.0.946 ±0.588,t =-5.680,P =0.000）;and the expre-ssion of miR-31 75 was obviously increased in glioma tissues （1 .008 ±0.355 vs.2.076 ±0.841 ,t =4.274, P =0.000）,and HOXB1 expression was negatively correlated with miR-31 75 expression in glioma tissues＆nbsp;（r =-0.601 ,P =0.000）.HOXB1 expression was related with histologic grade （χ2 =4.848,P =0.028）, and miR-31 75 expression was related with histologic grade （χ2 =5.640,P =0.01 8）and Karnofsky score （χ2 =4.785,P =0.029）.The results of Kaplan-Meier revealed that there were significant differences in median survival time between HOXB1 （or miR-31 75）high-expression group and HOXB1 （or miR-31 75）low-expression group [HOXB1 ：（21 .0 ±4.0）months vs.（7.0 ±0.8）months;χ2 =7.495,P =0.006;miR-31 75：（6.0 ±0.6）months vs.（1 6.0 ±5.8）months;χ2 =9.591 ,P =0.002].COX regression models showed that tumor degree （RR =6.556,95% CI：1 .1 96-35.952,P =0.002）,HOXB1 （RR =0.01 8, 95%CI：0.001 -0.31 2,P =0.006）and miR-31 75 （RR =2.098,95%CI：1 .663-7.51 3,P =0.037）were independent prognostic factors for prognosis.Conclusion The HOXB1 expression may be negatively correlated with miR-31 75 in human glioma tissues,and the expression levels of HOXB1 and miR-31 75 are associated with the glioma malignant degree,survival time and prognosis of glioma patients.
作者
周海霞
韩亮
祝子峰
魏君
田宇
李朝晖
Zhou Haixia Han Liang Zhu Zifeng Wei Jun Tian Yu Li Zhaohui(Special Ward, China-Japan Union Hospital of Jilin University, Changchun 130033, China)
出处
《国际肿瘤学杂志》
CAS
2016年第11期817-821,共5页
Journal of International Oncology
基金
吉林省科技发展计划国际科技合作项目（20130413028GH）
吉林省卫生计生青年科研基金（2015Q005）
