摘要
目的 观察微小RNA（miRNA,miR）-34a在乳腺癌中的表达以及对乳腺癌细胞生长、迁移和侵袭能力的影响.方法 采用反转录-聚合酶链反应（RT-PCR）法检测乳腺癌组织和正常乳腺组织中miR-34a的表达水平,分析miR-34a的表达与乳腺癌患者临床病理特征的相关性.在乳腺癌细胞MDA-MB-453中转染miR-34a模拟物和miR-34a抑制物,RT-PCR法检测转染后的乳腺癌细胞株中miR-34a表达水平,细胞计数试剂盒（CCK-8）法检测乳腺癌细胞的生长活性,Transwell法和细胞划痕实验分别检测乳腺癌细胞的迁移和侵袭能力.结果 miR-34a在乳腺癌组织和正常组织中的相对表达水平为0.433±0.059和1.040 ±0.072,两者差异有统计学意义（P＜0.05）,且miR-34a的表达水平与乳腺癌患者的临床分期和淋巴结转移有关.转染miR-34a模拟物后乳腺癌细胞MDA-MB-231的生长活性显著降低,MDA-MB-231和MCF-7细胞的迁移能力[（35.1±2.0）％、（28.7±1.8）％]和侵袭能力（41.6 ±3.9、39.4±4.3）显著降低,差异均有统计学意义（P＜0.05）.结论 miR-34a在乳腺癌中表达显著下调,其表达水平与乳腺癌患者的临床分期和淋巴结转移有关,过表达miR-34a可抑制乳腺癌细胞的生长、迁移和侵袭能力.
Objective To investigate the expression of microRNA （miRNA,miR）-34a in breast cancer and its influence on cell proliferation,cell invasiveness and migration capability of breast cancer cells.Methods The expression of miR-34a in breast cancer tissues and cells was measured by reverse transcriptase-polymerase chain reaction （RT-PCR） and the correlation of miR-34a expression and the clinical characteristics of breast cancer patients was analyzed.MiR-34a mimics and miR-34a inhibitor were transfected into breast cancer cells.The cell counting kit-8 （CCK-8） method was used to detect the cell proliferation of breast cancer cells,then we detected the cell migration and invasion ability of breast cancer cells transfected by miR-34a mimics and miR-34a inhibitor.Results The relative expression of miR-34a in breast cancer tissues and normal tissues was 0.433 ± 0.059 and 1.040 ± 0.072 respectively （P 〈 0.05）,and the expression level of miR-34a in breast cancer tissues was related to clinical stages and lymphatic metastasis.The overexpression of miR-34a in breast cancer cells could inhibit cell proliferation,migration [（35.1 ± 2.0） % and （28.7 ± 1.8） %] and invasion （41.6 ± 3.9 and 39.4 ± 4.3,P 〈0.05）.Conclusion miR-34a is down-regulated in breast cancer,and its expression level is related to clinical stages and lymphatic metastasis.MiR-34a may be involved in the progress of breast cancer by inhibiting the cell proliferation,migration and invasion of breast cancer cells.
作者
冯刚
刘志苏
Feng Gang Liu Zhisu(Department of General Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2016年第11期2513-2515,共3页
Chinese Journal of Experimental Surgery
