摘要
目的:研究新疆地区HPV16 E6、E7、LCR基因突变情况,分析HPV16变异体在宫颈癌及癌前病变发生发展中的作用。方法:选择HPV16阳性的宫颈癌及癌前病变患者,提取基因组DNA,利用PCR扩增HPV16 DNA E6、E7基因及LCR区核苷酸片段,正反向测序。与HPV16基因序列分析比对,分析核苷酸突变位点。结果:E6基因突变率为80.00%(92/115)主要突变位点T350G(59.78%)、T178G(18.47%);E7突变率为54.78%(63/115),主要突变位点A647G(33.33%)、T846C(26.98%);LCR突变率为23.48%(27/115),主要突变位点为C24T(74.07%)、C13T(25.92%)。维吾尔族T350G突变率较汉族妇女显著升高,而汉族A647G、T846C、C24T突变率显著高于维吾尔族,差异具有统计学意义(P<0.05)。维吾尔族宫颈癌组T350G突变率显著高于炎症组(P<0.05),且随病变严重程度增加突变率上升,汉族T350G、A647G、T846C、C24T突变率炎症组、宫颈病变组显著高于宫颈癌组(P<0.05),维吾尔族C24T突变率炎症组显著高于宫颈癌组(P<0.05),差异均具有统计学意义(P<0.05)。结论:HPV16E6、E7突变可能与宫颈病变进展有关,T350G突变可能是维吾尔族宫颈癌高发的原因之一。
Objective： To study E6, E7 and LCR gene mutation in Xinjiang; and analyze the effects of HPV16 variants in the occurrence and development of cervical cancer and precancerous lesions. Methods： Genomic DNA was extracted in patients with HPV16 positive cervical cancer and precancerous lesions. HPV16 DNA E6 and E7 genes and nucleotide fragments of LCR region were carried out the forward and reverse sequencing using PCR amplification. HPV16 pedigree distribution was confirmed via analyzing and comparing the HPV16 gene sequencing to analyze the mutation sites of nucleotide. Results： E6 gene mutation rate was 80.00%（92/115）and the main mutation sites were T350G（59.78%） and T178G（18.47%）. E7 gene mutation rate was 54.78%（63/115） and the main mutation sites were A647G（33.33%） and T846C（26.98%）. LCR mutation rate was 23.48%（27/115） and the main mutation sites were C24T（74.07%） and C13T（25.92%）. Compared the Uyghur women with Han women, Uyghur T350 G mutation rate was significantly higher. Han A647 G, T846 C and C24 T mutation rate were significantly higher than Uyghur（P 〈0.05）. T350 G mutation in the Uyghur cervical cancer group was significantly higher than that of inflammation group（P 〈0.05）. Moreover, the severity of mutation went up along with lesions. Han T350 G, A647 G, T846 C and C24 T mutation rates in the inflammation group and cervical lesions group were significantly higher than cervical cancer（P〈0.05）. Uyghur C24 T mutation rate in the inflammation group was significantly higher than cervical cancer group（P〈0.05）. Conclusion： HPV16E6 and E7 mutation might be related to cervical lesions. T350 G mutation might be the cause of the high incidence of cervical cancer in Uygur.
出处
《现代生物医学进展》
CAS
2016年第32期6244-6251,共8页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81450013
81360317)
新疆维吾尔自治区自然科学基金项目(2015211C208)
