摘要
目的:建立高效液相色谱法测定田七药材中的辅酶Q10的含量,为检测田七中的辅酶Q10提供新的方法。方法;色谱柱Eclipse Plus C18(4.6*250nm,5μm):流动相:甲醇:乙醇=(v:v)20:80:流速:1mL/min;检测波长:275nm;柱温:30℃;进样体积:20μL,结果:辅酶Q10在1.5625-25μg/mL范围内线性关系良好,平均回收率为62.92%,RSD%为6.24%。结论:该法快速、简便、灵敏、准确,适用于检测田七中的辅酶Q10。
Objective To develop a HPLC method for the determination of coenzyme Q10 in Panpax notoginseng. Methods Using Eclipse Plus C18 column, and kept pillar Temperature 30 centigrade, with Methanol : Ethanol (20 : 80) as the mobile phase, the flow rate was 1.0 milliliter per minute and detection wavelength was 275 nm. Results Coenzyme Q10 was lined in the concentration range of 1.5625-25 μg/mL. The average content of coenzyme Q10 in Panpax notoginseng was 62.92 % (RSD=6.24 %). Conclusion The method is sensitive, specific, reproducible and has good linearity. So it can be used to determine the content assay of coenzyme Q10 in Panpax notoginseng.
作者
丁鸿燕
吕思敏
吴铁
Ding Hongyan Lv Simin Wu Tie(Guangdong Medical University, Guangdong Runhe Biological Technology Co., Ltd., Coenzymc Q10 Research Institute, Dongguan 523808 School of Pharmacy, Guangdong Medical University, Dongguan 523808, China)
出处
《广东化工》
CAS
2016年第21期152-154,共3页
Guangdong Chemical Industry
基金
广东医科大学.广东润和生物科技有限公司共建辅酶Q10联合研究中心项目(No 2XX14002)