摘要
目的：了解寨卡病例的排毒途径和时间，为寨卡病例的实验检测提供科学依据。方法收集寨卡病例不同时间的多类临床样本，用real-time RT-PCR方法对其进行核酸检测，阳性标本进行乳鼠颅内和细胞接种分离寨卡病毒，将获得的寨卡病毒利用二代测序方法进行全基因组测序，所测得的序列与GenBank中寨卡序列进行系统进化分析。结果18例感染者尿液、唾液和血清样本中寨卡病毒核酸阳性率分别为82.4%（14/17）、82.4%（14/17）和52.9%（9/17），尿液排毒的持续时间最长，其次是唾液和血液。9份阳性血清标本共分离到6株寨卡病毒，进化分析6条寨卡基因组序列均属于亚洲族系，来自萨摩亚与委内瑞拉的病毒位于不同的进化分支中。结论唾液、血清和尿液均可用于寨卡病例的日常检测，尿液和唾液的核酸检测率更高，阳性血清更容易分离到寨卡病毒。乳鼠和细胞均可用于寨卡病毒的分离，但乳鼠颅内接种的病毒分离率更高。
Objective To provide scientific evidences for Zika virus detection by clarifying the means by which Zika virus was discharged and the duration of corresponding processes. Methods Various samples of Zika cases were collected at different times and detected by using real-time RT-PCR. The positive samples were inoculated into cells and suckling mice through intracranial injection. The whole genome se-quences of those isolated Zika virus strain were sequenced and the results were further analyzed by comparing with the sequences of Zika virus from GenBank. Results The positive rates of Zika virus in urine, saliva and serum samples were 82. 4% （14/17）, 82. 4% （14/17） and 52. 9% （9/17） respectively. The longest period of detected presence of Zika virus was found in urine samples amongst the three types of samples, followed by saliva and serum samples. Six Zika virus strains were isolated from 9 positive serum samples. Phy-logenetic analysis showed that the six genomes of Zika virus all belonged to Asia lineage, but located in two branches by Samoa and Venezuela strains. Conclusion This study indicated that urine, saliva and serum all could be used as the samples for routine detection of Zika virus. Urine and saliva samples showed higher detection rates of Zika virus RNA in comparison to serum samples, while Zika virus could be easily isolated from positive serum samples. Suckling mice were better for Zika virus isolation than cell lines.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2016年第10期721-726,共6页
Chinese Journal of Microbiology and Immunology
