摘要
目的研究不同剂量X射线照射人三阴性乳腺癌细胞,照射后不同时间点对人三阴性乳腺癌细胞E-钙黏蛋白(E-cadherin)基因表达的影响。方法用X射线分2.5、5和10 Gy 3个吸收剂量组照射体外培养的人三阴性乳腺癌HCC1937细胞和MDA-MB-231细胞,采用荧光实时定量PCR技术检测HCC1937细胞和MDA-MB-231细胞经照射后6、12、24和48 h的E-cadherin mRNA表达水平,以未照射组(0 h组)为对照。结果 HCC1937细胞和MDA-MB-231细胞E-cadherin mRNA的表达在2.5、5、10 Gy X射线照射后,随着时间点的后移呈升高趋势。与未照射组相比,除了HCC1937细胞10 Gy照射后6 h、2.5 Gy和5 Gy照射后24 h及MDA-MB-231细胞5 Gy照射后12 h外,各时间点差异均有统计学意义(P<0.05)。HCC1937细胞在2.5 Gy和10 Gy照射后48 h,E-cadherin mRNA的表达呈下降趋势,与未照射组相比,差异有统计学意义(P<0.05)。结论较大剂量的X射线整体可上调HCC1937细胞和MDA-MB-231细胞E-cadherin mRNA的表达,可能诱导了肿瘤细胞凋亡;晚期HCC1937细胞E-cadherin mRNA的表达下降,可能与辐射所致HCC1937细胞的放射生物学效应有关。
Objective To investigate the effects of different doses of X-ray irradiation on the expression of E-cadherin gene in triple negative breast cancer ceUs. Methods Human triple negative breast cancer MDA-MB-231 and HCC1937 cells were exposed to X-ray Irradiation at a dose of 2.5, 5 and 10 Gy for 6, 12, 24 and 48 h, respectively. E-cadherin mRNA expression levels in HCC1937 and MDA-MB-231 cells after irradiation were detected with fluorescence real-time PCR technology. Results E-cadherin mRNA expression levels in HCC1937 and MDA-MB-231 ceils after irradiation were raised with exposure time. Except HCC1937 cell 6 h after 10 Gy, 2.5 Gy irradiation and 24 h after 5 Gy irradiation, MDA- MB-231 cells 12 h after 5 Gy irradiation, there were significant differences at all time points between irradiation and non-irradiation groups （P 〈 0. 05 ）. E-cadherin mRNA expression of HCC1937 cells was decreased 48 h after 2.5 Gy and 10 Gy irradiation, compared with those of the non- irradiation group （ P 〈 0.05 ）. Conclusion High dose of X-rays irradiation may upgrade the expression of E-cadherin mRNA in human HCC1937 and MDA-MB-231cells, resulting in cell apoptosis. The decreased expression of E-cadherin mRNA in HCC1937 cells after long exposure to X-ray irradiation might be associated with the biological effect of radiation.
作者
张莹
王璐
刘利敏
张颖
石静
林清
ZHANG Ying WANG Lu LIU Li-min(Dept. of Tumor Radiotherapy, Tenth People＇s Hospital, Tongji University, Shanghai 200072, China Dept. General Surgery, Shanghai Baoshan District Hospital of Integrated Traditional Chinese Medicine with Western Medicine, Shanghai 201999, China)
出处
《同济大学学报（医学版）》
CAS
2016年第5期25-28,共4页
Journal of Tongji University(Medical Science)
