摘要
在4℃条件下,以精子活力为指标,研究了不同浓度的Na^+、K^+、Ca^(2+)、Mg^(2+)、葡萄糖、氨基酸等组成的5种(A、B、C、D、E)精子保存液及其适量添加青霉素对黑尾近红鲌(Ancherythroculter nigrocauda)精子活力的影响。结果显示:1)温度为4℃时,精子活力达80%的保存液A、B、C、D、E的保存时间分别为48、24、60、48、48 h,保存液C的保存时间明显高于其它各组;2)在各保存液中分别添加浓度为2.0×104IU/m L的青霉素,精子活力达80%的保存液A、B、C、D、E的保存时间分别为60、36、156、48、144 h,保存时间延长了12~96 h,保存液C和E的延长时间最长,均为96 h;3)人工授精试验证明,经保存的黑尾近红鲌精子能正常用于人工繁殖,受精率达(90.6±0.8)%^(91.8±0.9)%,与对照组精子受精率(92.4±0.8)%无显著差异。添加青霉素,保存液C的保存效果最好,其次是保存液E。
Under the condition of low temperature（ 4℃）,we studied the effects of 5 kinds of sperm preservation solutions（ A,B,C,D,E） containing different concentration of Na-＋,K-＋,Ca-（2＋）,Mg-（2＋）,amino acid,glucose and adding moderate amount of penicillin on the sperm motility in Ancherythroculter nigrocauda,to explored the best preservation condition of the sperm vitality as indicators. The results showed that： 1） Under the temperature of 4 ℃,the preservative solution of A,B,C,D and E could keep 80% of spermatozoa having fast motility within 48,24,60,48,48 h respectively,and the preservation time of C solution was obviously higher than that of other groups; 2） With the addition of 2. 0× 104 IU / m L penicillin,A,B,C,D and E could keep 80% of spermatozoa having fast motility within 60,36,156,48,144 h respectively,and the preservation time were prolonged for 12 h - 96 h. C and E solutions were extended to the longest time both with 96 h; 3） The experiment of artificial insemination proved that the preserved sperm of A. nigrocauda could be normally used for artificial breeding,and the fertilization rate（ 90. 6 ± 0. 8） % -（ 91. 8 ± 0. 9） %had no significant difference（ p〈0. 05） compared with control group fresh sperm（ 92. 4 ± 0. 8%）. The results indicated that among the preservation solutions with addition of 2. 0 × 104 IU / m L penicillin,the C solution had the best preservation effect,followed by E,which could be applied in the factory production.
出处
《淡水渔业》
CSCD
北大核心
2016年第6期3-7,32,共6页
Freshwater Fisheries
基金
国家科技计划项目(2015BAD25B01-5)
武汉市黄鹤英才计划项目(武人才办[2014]8号
序号19)
武汉市科技计划项目(20032003035
2013020705070350-15
2014020202010137)
